McqMate
These multiple-choice questions (MCQs) are designed to enhance your knowledge and understanding in the following areas: Bachelor of Agriculture (BSc agri) .
Chapters
| 1. |
The genetic material in HIV is |
| A. | ds DNA |
| B. | ss DNA |
| C. | s RNA |
| D. | None of these |
| Answer» A. ds DNA | |
| 2. |
Which one of the following mutagens act only on replicating DNA? |
| A. | Ethidium bromide |
| B. | Nitrosogeranidine |
| C. | Acridine orange |
| D. | None of above |
| Answer» C. Acridine orange | |
| 3. |
Poly A tail is frequently found in |
| A. | Histone in RNA |
| B. | Bacterial RNA |
| C. | eukaryotic RNA |
| D. | TRNA |
| Answer» C. eukaryotic RNA | |
| 4. |
Which of the following is an example of RNA virus? |
| A. | SV 40 |
| B. | T4 phage |
| C. | Tobacco mosaic virus |
| D. | Adeno virus |
| Answer» C. Tobacco mosaic virus | |
| 5. |
Genomic DNA is extracted, broken into fragments of reasonable size by a restriction endonuclease and then inserted into a cloning vector to generate chimeric vectors. The cloned fragments are called |
| A. | Clone |
| B. | Genomic library |
| C. | mRNA |
| D. | None of these |
| Answer» B. Genomic library | |
| 6. |
Transgenic animals are produced when GH gene fused with |
| A. | MT gene |
| B. | GH |
| C. | GRF |
| D. | FIX |
| Answer» A. MT gene | |
| 7. |
In which medium the hydridoma cells grow selectively? |
| A. | Polyethylene glycol |
| B. | Hypoxanthine aminopterin thyminine |
| C. | Hypoxathing-guaning phosphoribosyl transferase |
| D. | Both b and c |
| Answer» B. Hypoxanthine aminopterin thyminine | |
| 8. |
The enzymes which are commonly used in genetic engineering are |
| A. | Exonuclease and ligase |
| B. | Restriction endonuclease and polymerase |
| C. | Ligase and polymerase |
| D. | Restriction endonuclease and ligase |
| Answer» A. Exonuclease and ligase | |
| 9. |
A successful hybridoma was produced by fusing |
| A. | Plasma cells and plasmids |
| B. | Plasma cells and myeloma cells |
| C. | Myeloma cells and plasmids |
| D. | Plasma cells and bacterial cells |
| Answer» B. Plasma cells and myeloma cells | |
| 10. |
The technique involved in comparing the DNA components of two samples is known as |
| A. | Monoclonal antibody techniques |
| B. | Genetic finger printing |
| C. | Recombinant DNA technology |
| D. | Polymerase chain reaction |
| Answer» B. Genetic finger printing | |
| 11. |
Plasmids are ideal vectors for gene cloning as |
| A. | They can be multiplied by culturing |
| B. | They can be multiplied in the laboratory using enzymes |
| C. | They can replicate freely outside the bacterial cell |
| D. | They are self replicating within the bacterial cell |
| Answer» D. They are self replicating within the bacterial cell | |
| 12. |
Humans normally have 46 chromosomes in skin cells. How many autosomes would be expected in a kidney cell? |
| A. | 46 |
| B. | 23 |
| C. | 47 |
| D. | 44 |
| Answer» D. 44 | |
| 13. |
Pasteur effect is due to |
| A. | Change from aerobic to anaerobic |
| B. | Providing oxygen to anaerobically respiring structures |
| C. | Rapid utilization of ATP |
| D. | Nonsynthesis of ATP |
| Answer» B. Providing oxygen to anaerobically respiring structures | |
| 14. |
A mechanism that can cause a gene to move from one linkage group to another is |
| A. | Trans location |
| B. | Inversion |
| C. | Crossing over |
| D. | Duplication |
| Answer» A. Trans location | |
| 15. |
The smallest unit of genetic material that can undergo mutation is called |
| A. | Gene |
| B. | Cistron |
| C. | Replicon |
| D. | Muton |
| Answer» D. Muton | |
| 16. |
The two chromat ids of metaphase chrosome represent |
| A. | Replicated chromosomes to be separated at anaphase |
| B. | Homologous chromosomes of a diploid set |
| C. | Non-homologous chromosomes joined at the centromere |
| D. | Maternal and paternal chromosomes joined |
| Answer» A. Replicated chromosomes to be separated at anaphase | |
| 17. |
Malate dehydrogenase enzyme is a |
| A. | Transferase |
| B. | Hydrolase |
| C. | Isomerase |
| D. | Oxido reductase |
| Answer» D. Oxido reductase | |
| 18. |
In E.Coli att site is in between |
| A. | Gal and biogenes |
| B. | Bio and niacin genes |
| C. | Gal and B genes |
| D. | None of these |
| Answer» A. Gal and biogenes | |
| 19. |
The best vector for gene cloning |
| A. | Relaxed control plasmid |
| B. | Stringent control plasmid |
| C. | Both a and b |
| D. | None of these |
| Answer» A. Relaxed control plasmid | |
| 20. |
A gene that takes part in the synthesis of polypeptide is |
| A. | Structural gene |
| B. | Regulator gene |
| C. | Operator gene |
| D. | Promoter gene |
| Answer» A. Structural gene | |
| 21. |
DNA replicates during |
| A. | G1 – phase |
| B. | S – phase |
| C. | G2 – phase |
| D. | M – phase |
| Answer» B. S – phase | |
| 22. |
A human cell containing 22 autosome and a ‘Y’ chromosome is probably a |
| A. | Male somatic cell |
| B. | Zygote |
| C. | Female somatic cell |
| D. | Sperm cell |
| Answer» B. Zygote | |
| 23. |
Crossing-over most commonly occurs during |
| A. | Prophase I |
| B. | Prophase II |
| C. | Anaphase I |
| D. | Telophase II |
| Answer» A. Prophase I | |
| 24. |
DNA-replication is by the mechanism of |
| A. | Conservative |
| B. | Semiconservative |
| C. | Dispersive |
| D. | None of the above |
| Answer» B. Semiconservative | |
| 25. |
Production of RNA from DNA is called |
| A. | Translation |
| B. | RNA splicing |
| C. | Transcription |
| D. | Transposition |
| Answer» C. Transcription | |
| 26. |
Nucleic acids contain |
| A. | Alanine |
| B. | Adenine |
| C. | Lysine |
| D. | Arginine |
| Answer» B. Adenine | |
| 27. |
What are the structural units of nucleic acids? |
| A. | N-base |
| B. | Nucleosides |
| C. | Nucleotides |
| D. | Histones |
| Answer» C. Nucleotides | |
| 28. |
The most important function of a gene is to synthesize |
| A. | Enzyme |
| B. | Hormones |
| C. | RNA |
| D. | DNA |
| Answer» A. Enzyme | |
| 29. |
One of the genes present exclusively on the X-chromosome in humans is concerned with |
| A. | Baldness |
| B. | Red-green colour baldness |
| C. | Facial hair/moustache in males |
| D. | Night blindness |
| Answer» B. Red-green colour baldness | |
| 30. |
Peptide linkages are formed in between |
| A. | Nucleotides |
| B. | Amino acids |
| C. | Glucose molecules |
| D. | Sucrose |
| Answer» B. Amino acids | |
| 31. |
The nucleic acid of polio viruses is |
| A. | DNA |
| B. | RNA – (+) type |
| C. | t-RNA |
| D. | m-RNA |
| Answer» B. RNA – (+) type | |
| 32. |
Rabies virus is |
| A. | Nake RNA virus |
| B. | Naked DNA virus |
| C. | Enveloped RNA virus |
| D. | Enveloped DNA virus |
| Answer» C. Enveloped RNA virus | |
| 33. |
Example for DNA virus: |
| A. | Polio viru |
| B. | Adeno virus |
| C. | Echo virus |
| D. | Poty virus |
| Answer» B. Adeno virus | |
| 34. |
In genetic engineering breaks in DNA are formed by enzymes known as |
| A. | Restriction enzymes |
| B. | Ligases |
| C. | Nucleases |
| D. | Hydralases |
| Answer» B. Ligases | |
| 35. |
DNA transfer from one bacterium to another through phages is termed as |
| A. | Transduction |
| B. | Induction |
| C. | Transfection |
| D. | Infection |
| Answer» A. Transduction | |
| 36. |
Microorganisms usually make acetyl CO-A by oxidizing |
| A. | Acetic acid |
| B. | Pyruvic acid |
| C. | α-ketoglutaric acid |
| D. | Fumaric acid |
| Answer» A. Acetic acid | |
| 37. |
The method of DNA replication proposed by Watson and Crick is |
| A. | Semi conservative |
| B. | Conservative |
| C. | Dispersive |
| D. | Rolling loop |
| Answer» A. Semi conservative | |
| 38. |
The distance between each turn in the helical strand of DNA is |
| A. | 20 Ao |
| B. | 34 Ao |
| C. | 28 Ao |
| D. | 42 Ao |
| Answer» B. 34 Ao | |
| 39. |
Self-replicating, small circular DNA molecules present in bacterial cell are known |
| A. | Plasmid |
| B. | Cosmids |
| C. | Plasmomeros |
| D. | plastides |
| Answer» A. Plasmid | |
| 40. |
Western blotting is the technique used in the determination of |
| A. | RNA |
| B. | DNA |
| C. | Proteins |
| D. | All of these |
| Answer» B. DNA | |
| 41. |
m RNA synthesis from DNA is termed |
| A. | Transcription |
| B. | Transformation |
| C. | Translation |
| D. | Replication |
| Answer» A. Transcription | |
| 42. |
Western blotting is a technique used in the determination of |
| A. | DNA |
| B. | RNA |
| C. | Protein |
| D. | Polysaccharides |
| Answer» A. DNA | |
| 43. |
Building blocks of Nucleic acids are |
| A. | Amino acid |
| B. | Nucleosides |
| C. | Nucleotides |
| D. | Nucleo proteins |
| Answer» C. Nucleotides | |
| 44. |
DNA finger printing is based on |
| A. | Repetitive sequences |
| B. | Unique sequences |
| C. | Amplified sequences |
| D. | Non-coding sequences |
| Answer» B. Unique sequences | |
| 45. |
The enzyme required for DNA from RNA template: |
| A. | RNA polymerase |
| B. | Reverse transcriptase |
| C. | DNA polymerase |
| D. | Terminal transferase |
| Answer» B. Reverse transcriptase | |
| 46. |
Double standard RNA is seen in |
| A. | Reo viru |
| B. | Rhabdo virus |
| C. | Parvo virus |
| D. | Retro virus |
| Answer» A. Reo viru | |
| 47. |
The two strands of DNA are joined noncovalently by |
| A. | Ionic bonds |
| B. | Covalent bonds |
| C. | Hydrogen bonds between bases |
| D. | Polar charges |
| Answer» C. Hydrogen bonds between bases | |
| 48. |
The bases Adenine and Thymine are paired with |
| A. | Double hydrogen bonds |
| B. | Single hydrogen bonds |
| C. | Triple hydrogen bonds |
| D. | Both b and c |
| Answer» A. Double hydrogen bonds | |
| 49. |
The no. of hydrogen bonds existing between Guanine and Cytosine are |
| A. | 5 |
| B. | 2 |
| C. | 3 |
| D. | None of these |
| Answer» C. 3 | |
| 50. |
The length of each coil in DNA strand is |
| A. | 15 Ao |
| B. | 34 Ao |
| C. | 30 Ao |
| D. | 5 Ao |
| Answer» B. 34 Ao | |
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