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990+ Microbiology Solved MCQs

These multiple-choice questions (MCQs) are designed to enhance your knowledge and understanding in the following areas: Bachelor of Agriculture (BSc agri) .

Chapters

Chapter: Sterilisation, Culture Media and Pure Culture Techniques
401.

Temperature used for hot air oven is

A. 100oC for 1 hour
B. 120oC for 1 hour
C. 160oC for 1 hour
D. 60oC for 1 hour
Answer» C. 160oC for 1 hour
402.

Phenol co-efficient indicates

A. Efficiency of a disinfectant
B. Dilution of a disinfectant
C. Purity of a disinfectant
D. Quantity of a disinfectant
Answer» A. Efficiency of a disinfectant
403.

This is an agar plate method and is commonly used for estimation of the number of bacteria in milk.

A. Standard Plate Count (SPC)
B. Spread plate
C. Lawn culture
D. Roll tube method
Answer» A. Standard Plate Count (SPC)
404.

Agar is obtained form

A. Brown algae
B. Red algae
C. Green algae
D. Blue-green algae
Answer» B. Red algae
405.

A gram positive organism which produces swarming on culture medium is

A. Salmonella
B. Clostridium
C. Staphylococci
D. Proteus
Answer» D. Proteus
406.

Enhancement of virulence in bacteria is known as

A. Pathogenicity
B. Attenuation
C. Exaltation
D. Toxigenicity
Answer» C. Exaltation
407.

For effective sterilization in an autoclave the temperature obtained is

A. 50oC
B. 100oC
C. 120oC
D. 180oC
Answer» C. 120oC
408.

Spores are killed by

A. 70% alcohol
B. Glutaraldehyde
C. Autoclaving
D. Both b and c
Answer» D. Both b and c
409.

Glassware are sterilized by

A. Autoclaving
B. Hot air over
C. Incineration
D. None of these
Answer» B. Hot air over
410.

Tyndallisation was proposed by

A. Tyndall
B. Pasteur
C. Koch
D. Jenner
Answer» A. Tyndall
411.

Viruses can be cultivated in

A. Lab media
B. Broth
C. Living cells
D. None of these
Answer» C. Living cells
412.

By pasteurization

A. All the microorganisms can be removed
B. Only pathogenic forms can be removed
C. Only non-pathogenic forms can be removed
D. All of these are correct
Answer» B. Only pathogenic forms can be removed
413.

The temperature required for pasteurization is

A. Above 100oC
B. Below 100oC
C. 100oC
D. None of these
Answer» B. Below 100oC
414.

In the medium other than nutrients, if any substance is used in excess, that medium is

A. Enriched medium
B. Special medium
C. Enrichment medium
D. None of these
Answer» A. Enriched medium
415.

Example for indicator medium is

A. Nutrient Agar
B. Nutrient broth
C. Wilson and Blair
D. Czapeck-dox medium
Answer» C. Wilson and Blair
416.

Example of Anaerobic medium is

A. Robertson cooked-meat medium
B. Nutrient agar
C. Nutrient broth
D. Mac-Conkey’s agar
Answer» A. Robertson cooked-meat medium
417.

The differentiate lactose and non-lactose fermentors, the medium used is

A. Wilson & lair
B. Blood Agar
C. Tetra thionate broth
D. Mac-Conkey’s Agar
Answer» D. Mac-Conkey’s Agar
418.

Best method for getting pure culture is

A. Streak-plate
B. Agar slant
C. Both a & b
D. None of these
Answer» C. Both a & b
419.

To transfer cultures from one place to another, the device used is

A. Slant
B. Needle
C. Inoculation loop
D. Autoclave
Answer» B. Needle
420.

The bacterial culture prepared by pure culture is

A. Inoculum
B. Suspension
C. Dilution
D. None of these
Answer» A. Inoculum
421.

Separation of a single colony is

A. Pure-culturing
B. Isolation
C. Separation
D. Both a and b
Answer» B. Isolation
422.

Growth period of the culture is

A. Inoculation
B. Incubation
C. Incineration
D. Isolation
Answer» B. Incubation
423.

At the temperature 160oC for one hour, complete sterilization occurs in

A. Autoclave
B. Hot air oven
C. Laminar flow
D. Incubator
Answer» B. Hot air oven
424.

In autoclave, the principle involved is

A. Dry heat
B. Moist heat
C. Steam under pressur
D. Both b and c
Answer» D. Both b and c
425.

The spores of th bacteria which can withstand the moist heat effect also

A. Bacillus subtilis
B. Coxiella burnetti
C. Bacillus stearothermophilus
D. Pseudomonas
Answer» C. Bacillus stearothermophilus
426.

Factors on which disinfectivity of a disinfectant depends

A. Concentration of the substance
B. Time of action
C. pH of the medium and temperature suitable for the chemical
D. All of the above
Answer» D. All of the above
427.

Aldehydes, which are most powerful disinfectants

A. Formaldehyde
B. Acetaldehyde
C. Glutamal aldehyde
D. Both a and c
Answer» D. Both a and c
428.

Accridine dyes are more effective against

A. Gram positive
B. Gram negative
C. Mycoplasmas
D. Rickttsiae
Answer» A. Gram positive
429.

The sterilizing agent is

A. Ethelene oxide
B. Oxygen
C. Nitrogen
D. Carbon tetrachloride
Answer» A. Ethelene oxide
430.

Salts of heavy metals used as disinfectants are

A. Thiomersal
B. Phenyl mercury nitrate
C. Mercurochrome
D. All of these
Answer» D. All of these
431.

Cultures are prepared by penetrating the inoculation loop with suspension into the medium, they are

A. Stock culture
B. Stabcultures
C. Sub-cultures
D. None of these
Answer» B. Stabcultures
432.

The principle involved in the streak plate method is

A. Separation
B. Streaking
C. Isolation
D. Dilution
Answer» D. Dilution
433.

Culture media for fungi are

A. Potato dextrose agar (PDA)
B. Sabouraud’s agar
C. Czapekdox agar
D. All of the above
Answer» D. All of the above
434.

Spores of actinomycetes are very sensitive, killed at room temperature of

A. 52oC for 30 min.
B. 65oC for 30 min.
C. 70oC for 30 min.
D. 43oC for 30 min.
Answer» B. 65oC for 30 min.
435.

The term that is used for the bacteria which can withstand pasteurization but does not grow at higher temperatures

A. Thermophiles
B. Extreme thermophiles
C. Thermoduric
D. Facultative thermophiles
Answer» C. Thermoduric
436.

A common laboratory method of cultivating anaerobic micro-organisms is

A. Gas pack system
B. Brewer jar system
C. Pyrogallic acid over the cotton
D. None of these
Answer» C. Pyrogallic acid over the cotton
437.

Alkaliphiles grow at pH value between

A. 1 to 6
B. 6 to 9
C. 1 to 11
D. 7 to 12
Answer» D. 7 to 12
438.

The micro-organisms grow at high salinity are

A. Osmophile
B. Halophiles
C. Both a and b
D. None of these
Answer» C. Both a and b
439.

Non-lactose fermenting colonies seen on Mac Conkey’s medium are

A. Salmonella typhi
B. Escherichia coli
C. Klebsiella pneumoniae
D. Shigella shigae
Answer» A. Salmonella typhi
440.

Wilson and Blair medium is used for isolation of

A. Staphylococci
B. Salmonella typhosa
C. Vibrio cholerae
D. Shigella shigae
Answer» B. Salmonella typhosa
441.

Laboratory diagnosis of enteric fever is based on

A. Blood culture
B. Urine and stool culture
C. Widal test
D. All of the above
Answer» D. All of the above
442.

Shigella was first isolated by

A. Shiga
B. Schmitz
C. Sonnei
D. Robert Koch
Answer» C. Sonnei
443.

Which of the following are gas producing Salmonella?

A. S.typhi
B. S.enteritidis
C. S.cholerasuis
D. S.typhimurium
Answer» B. S.enteritidis
444.

Kauffmann white scheme is used to detect

A. Salmonella spp.
B. Shigella spp.
C. E.coli
D. None of these
Answer» A. Salmonella spp.
445.

On Mac Conkey’s medium Esch. Coli forms

A. Colorless colonies
B. Greenish pigmentation
C. Pink coloured colonies
D. Medusa head appearance
Answer» C. Pink coloured colonies
446.

C.diphtheriae requires

A. LJ medium
B. Mac Conkey’s medium
C. Potassium tellurite medium
D. PDA medium
Answer» C. Potassium tellurite medium
447.

Culture medium for Mycobacterium tuberculosis

A. LJ medium
B. Mac Conkey’s medium
C. Wilson blair medium
D. None of these
Answer» A. LJ medium
448.

Lepra bacillus is best cultured on

A. Armadillo’s brain
B. Foot pad of mice
C. Liver of guinea pig
D. Any of the above
Answer» B. Foot pad of mice
449.

Culture medium for clostridia spp.

A. 76 Lower stein Jensen’s medium
B. Mac Conkey’s medium
C. Robertson’s cooked meat medium
D. None of these
Answer» C. Robertson’s cooked meat medium
450.

Clsotridium welchii is positive for

A. Elek’s gel precipitation test
B. Nagler’s test
C. Weil felix test
D. Bacitracin test
Answer» B. Nagler’s test

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