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Chapter:

90+ Sterilisation, Culture Media and Pure Culture Techniques Solved MCQs

in Microbiology

These multiple-choice questions (MCQs) are designed to enhance your knowledge and understanding in the following areas: Bachelor of Agriculture (BSc agri) .

Chapters

Chapter: Sterilisation, Culture Media and Pure Culture Techniques
51.

The bacterial culture prepared by pure culture is

A. Inoculum
B. Suspension
C. Dilution
D. None of these
Answer» A. Inoculum
52.

Separation of a single colony is

A. Pure-culturing
B. Isolation
C. Separation
D. Both a and b
Answer» B. Isolation
53.

Growth period of the culture is

A. Inoculation
B. Incubation
C. Incineration
D. Isolation
Answer» B. Incubation
54.

At the temperature 160oC for one hour, complete sterilization occurs in

A. Autoclave
B. Hot air oven
C. Laminar flow
D. Incubator
Answer» B. Hot air oven
55.

In autoclave, the principle involved is

A. Dry heat
B. Moist heat
C. Steam under pressur
D. Both b and c
Answer» D. Both b and c
56.

The spores of th bacteria which can withstand the moist heat effect also

A. Bacillus subtilis
B. Coxiella burnetti
C. Bacillus stearothermophilus
D. Pseudomonas
Answer» C. Bacillus stearothermophilus
57.

Factors on which disinfectivity of a disinfectant depends

A. Concentration of the substance
B. Time of action
C. pH of the medium and temperature suitable for the chemical
D. All of the above
Answer» D. All of the above
58.

Aldehydes, which are most powerful disinfectants

A. Formaldehyde
B. Acetaldehyde
C. Glutamal aldehyde
D. Both a and c
Answer» D. Both a and c
59.

Accridine dyes are more effective against

A. Gram positive
B. Gram negative
C. Mycoplasmas
D. Rickttsiae
Answer» A. Gram positive
60.

The sterilizing agent is

A. Ethelene oxide
B. Oxygen
C. Nitrogen
D. Carbon tetrachloride
Answer» A. Ethelene oxide
61.

Salts of heavy metals used as disinfectants are

A. Thiomersal
B. Phenyl mercury nitrate
C. Mercurochrome
D. All of these
Answer» D. All of these
62.

Cultures are prepared by penetrating the inoculation loop with suspension into the medium, they are

A. Stock culture
B. Stabcultures
C. Sub-cultures
D. None of these
Answer» B. Stabcultures
63.

The principle involved in the streak plate method is

A. Separation
B. Streaking
C. Isolation
D. Dilution
Answer» D. Dilution
64.

Culture media for fungi are

A. Potato dextrose agar (PDA)
B. Sabouraud’s agar
C. Czapekdox agar
D. All of the above
Answer» D. All of the above
65.

Spores of actinomycetes are very sensitive, killed at room temperature of

A. 52oC for 30 min.
B. 65oC for 30 min.
C. 70oC for 30 min.
D. 43oC for 30 min.
Answer» B. 65oC for 30 min.
66.

The term that is used for the bacteria which can withstand pasteurization but does not grow at higher temperatures

A. Thermophiles
B. Extreme thermophiles
C. Thermoduric
D. Facultative thermophiles
Answer» C. Thermoduric
67.

A common laboratory method of cultivating anaerobic micro-organisms is

A. Gas pack system
B. Brewer jar system
C. Pyrogallic acid over the cotton
D. None of these
Answer» C. Pyrogallic acid over the cotton
68.

Alkaliphiles grow at pH value between

A. 1 to 6
B. 6 to 9
C. 1 to 11
D. 7 to 12
Answer» D. 7 to 12
69.

The micro-organisms grow at high salinity are

A. Osmophile
B. Halophiles
C. Both a and b
D. None of these
Answer» C. Both a and b
70.

Non-lactose fermenting colonies seen on Mac Conkey’s medium are

A. Salmonella typhi
B. Escherichia coli
C. Klebsiella pneumoniae
D. Shigella shigae
Answer» A. Salmonella typhi
71.

Wilson and Blair medium is used for isolation of

A. Staphylococci
B. Salmonella typhosa
C. Vibrio cholerae
D. Shigella shigae
Answer» B. Salmonella typhosa
72.

Laboratory diagnosis of enteric fever is based on

A. Blood culture
B. Urine and stool culture
C. Widal test
D. All of the above
Answer» D. All of the above
73.

Shigella was first isolated by

A. Shiga
B. Schmitz
C. Sonnei
D. Robert Koch
Answer» C. Sonnei
74.

Which of the following are gas producing Salmonella?

A. S.typhi
B. S.enteritidis
C. S.cholerasuis
D. S.typhimurium
Answer» B. S.enteritidis
75.

Kauffmann white scheme is used to detect

A. Salmonella spp.
B. Shigella spp.
C. E.coli
D. None of these
Answer» A. Salmonella spp.
76.

On Mac Conkey’s medium Esch. Coli forms

A. Colorless colonies
B. Greenish pigmentation
C. Pink coloured colonies
D. Medusa head appearance
Answer» C. Pink coloured colonies
77.

C.diphtheriae requires

A. LJ medium
B. Mac Conkey’s medium
C. Potassium tellurite medium
D. PDA medium
Answer» C. Potassium tellurite medium
78.

Culture medium for Mycobacterium tuberculosis

A. LJ medium
B. Mac Conkey’s medium
C. Wilson blair medium
D. None of these
Answer» A. LJ medium
79.

Lepra bacillus is best cultured on

A. Armadillo’s brain
B. Foot pad of mice
C. Liver of guinea pig
D. Any of the above
Answer» B. Foot pad of mice
80.

Culture medium for clostridia spp.

A. 76 Lower stein Jensen’s medium
B. Mac Conkey’s medium
C. Robertson’s cooked meat medium
D. None of these
Answer» C. Robertson’s cooked meat medium
81.

Clsotridium welchii is positive for

A. Elek’s gel precipitation test
B. Nagler’s test
C. Weil felix test
D. Bacitracin test
Answer» B. Nagler’s test
82.

Nagler’s reaction detects

A. Coagulase
B. Hyaluronidase
C. Lecithinase
D. None of these
Answer» C. Lecithinase
83.

Incubation period of Cl. welchii is

A. 8-12 hour
B. 7-10 hours
C. 5-7 hours
D. 2-4 hours
Answer» A. 8-12 hour
84.

The average incubation period of tetanus is

A. 2-3 day
B. 7-10 days
C. 14-21 days
D. 3-4 weeks
Answer» B. 7-10 days
85.

Salt agar is used for

A. Streptococcu
B. Staphylococcus
C. Vibrio
D. Shigella
Answer» B. Staphylococcus
86.

Culture medium of Leishmania is

A. Sabousand’s medium
B. NNN medium
C. Wilson Blair medium
D. Czapek – dox medium
Answer» B. NNN medium
87.

A simple asexual spore which develops by budding is known as

A. Chlamydospore
B. Blastospore
C. Arthospore
D. Conidia
Answer» B. Blastospore
88.

Culture medium used for fungus is

A. Sabouraud’s medium
B. Nutrient agar
C. Nutrient broth
D. Minimal agar medium
Answer» A. Sabouraud’s medium
89.

For ster i l izat ion of fermentat ion equipment the method followed is

A. Radiation
B. Chemicals
C. Heating
D. All of these
Answer» D. All of these
90.

The size of the virus can be determined by

A. Micrography
B. Ultra-centrifugation at high speed
C. Ultra-filteration
D. All of these
Answer» D. All of these
91.

Differential staining of bacteria spore is related to

A. Albert’s staining
B. Lugol’ s staining
C. Moller’s staining
D. Indian ink preparation
Answer» C. Moller’s staining
92.

Wilson and Blair bismuth sulphite medium is used for the growth

A. Salmonella typhi
B. Shigella dysenteriae
C. Vibrio cholerae
D. E. coli
Answer» A. Salmonella typhi
93.

Which Rickettsia can be grown on blood agar media?

A. Lactobacilli
B. Streptobacillus
C. Bacillus anthrax
D. Vibrio cholerae
Answer» A. Lactobacilli

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