McqMate
These multiple-choice questions (MCQs) are designed to enhance your knowledge and understanding in the following areas: Bachelor of Pharmacy (B. Pharma) .
| 101. |
Which one of the following will travel fastest through the gel if the amount of DNA present is same in all? |
| A. | Circular |
| B. | Supercoiled |
| C. | Nicked |
| D. | Supercoiled and circular will move at same speed and faster than nicked |
| Answer» B. Supercoiled | |
| 102. |
How is the size of molecules under analysis measured? |
| A. | By measuring the distance moved through a ruler |
| B. | By measuring the amount of visualising dye used |
| C. | By running a standard molecule, whose size is known in parallel |
| D. | There is no exact criterion for doing so |
| Answer» C. By running a standard molecule, whose size is known in parallel | |
| 103. |
Gel matrices are generally of two types Agarose and Polyacrylamide. Which of the statements is correct with respect to agarose gels. |
| A. | Agarose is a polysaccharide which is obtained from red algae |
| B. | Agarose is a polysaccharide obtained from fungus |
| C. | It is composed of glucose residues |
| D. | It is obtained via dissolving in its water by boiling in water and then cooling it |
| Answer» A. Agarose is a polysaccharide which is obtained from red algae | |
| 104. |
Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels? |
| A. | They are obtained via polymerization between acrylamide and bis-acrylamide |
| B. | The components added for initiating polymerization are ammonium persulphate and TEMED |
| C. | It is casted in horizontal and flat trays |
| D. | TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking |
| Answer» C. It is casted in horizontal and flat trays | |
| 105. |
If the amount of agarose added is more, the molecular under analysis should have the following characteristic……………….. |
| A. | small size |
| B. | large size |
| C. | size has no relation with the amount of agarose |
| D. | the amount of molecules under analysis matters |
| Answer» A. small size | |
| 106. |
In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used? |
| A. | Renaturing |
| B. | Denaturing |
| C. | The routine agarose gel |
| D. | The routine polyacrylamide gel |
| Answer» B. Denaturing | |
| 107. |
The technique used to located specific genes in chromosomes is …………….. |
| A. | Colony Hybridization |
| B. | In situ Hybridization |
| C. | Dot Blot Hybridization |
| D. | Western Blotting |
| Answer» B. In situ Hybridization | |
| 108. |
The separation of charged molecules under the influence of electric current is called………. |
| A. | Colony Hybridization |
| B. | Dot Blot Hybridization |
| C. | Electrophoresis |
| D. | Western Blotting |
| Answer» C. Electrophoresis | |
| 109. |
If further electrophoresis is used for recovery of DNA from gels, the method is termed as ____________ |
| A. | secondary electrophoresis |
| B. | recovery electrophoresis |
| C. | denaturing electrophoresis |
| D. | electro-elution |
| Answer» D. electro-elution | |
| 110. |
The greatest separation is obtained in which portion of the gel? |
| A. | Lower portion where the anode is |
| B. | Lower portion where the cathode is |
| C. | The separation is uniform all over |
| D. | It varies according to the quantity of the size of the molecules to be separated |
| Answer» A. Lower portion where the anode is | |
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