McqMate
These multiple-choice questions (MCQs) are designed to enhance your knowledge and understanding in the following areas: Uncategorized topics .
| 1. |
Restriction enzymes were discovered by |
| A. | Smith and Nathans |
| B. | Alexander Fleming |
| C. | Berg |
| D. | None |
| Answer» A. Smith and Nathans | |
| 2. |
Bacteria protect themselves from viruses by fragmenting viral DNA with |
| A. | Ligase |
| B. | Endonuclease |
| C. | Exonuclease |
| D. | Gyrase |
| Answer» B. Endonuclease | |
| 3. |
Klenow fragment is derived from |
| A. | DNA Ligase |
| B. | DNA Pol-I |
| C. | DNA Pol-II |
| D. | Reverse Transcriptase |
| Answer» B. DNA Pol-I | |
| 4. |
Southern blotting is |
| A. | Attachment of probes to DNA fragments |
| B. | Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet |
| C. | Comparison of DNA fragments to two sources |
| D. | Transfer of DNA fragments to electrophoretic gel from cellulose membrane |
| Answer» B. Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet | |
| 5. |
ELISA is |
| A. | Using radiolabelled second antibody |
| B. | Usage of RBCs |
| C. | Using complement-mediated cell lysis |
| D. | Addition of substrate that is converted into a coloured end product |
| Answer» D. Addition of substrate that is converted into a coloured end product | |
| 6. |
The Golden Rice variety is rich in |
| A. | Vitamin C |
| B. | Β-carotene and ferritin |
| C. | Biotin |
| D. | Lysine |
| Answer» B. Β-carotene and ferritin | |
| 7. |
The DNA fragments have sticky ends due to |
| A. | Endonuclease |
| B. | Unpaired bases |
| C. | Calcium ions |
| D. | Free methylation |
| Answer» B. Unpaired bases | |
| 8. |
Plasmids are used as cloning vectors for which of the following reasons? |
| A. | Can be multiplied in culture |
| B. | Self-replication in bacterial cells |
| C. | Can be multiplied in laboratories with the help of enzymes |
| D. | Replicate freely outside bacterial cells |
| Answer» B. Self-replication in bacterial cells | |
| 9. |
The human genome project was launched in the year |
| A. | 1980 |
| B. | 1973 |
| C. | 1990 |
| D. | 1989 |
| Answer» C. 1990 | |
| 10. |
The vaccines prepared through recombinant DNA technology are |
| A. | Third generation vaccines |
| B. | First-generation vaccines |
| C. | Second-generation vaccines |
| D. | None |
| Answer» A. Third generation vaccines | |
| 11. |
Which is a genetically modified crop? |
| A. | Bt-cotton |
| B. | Bt-brinjal |
| C. | Golden rice |
| D. | All |
| Answer» D. All | |
| 12. |
PCR technique was invented by |
| A. | Karry Mullis |
| B. | Boyer |
| C. | Sanger |
| D. | Cohn |
| Answer» A. Karry Mullis | |
| 13. |
The first transgenic plant to be produced is |
| A. | Brinjal |
| B. | Tobacco |
| C. | Rice |
| D. | Cotton |
| Answer» B. Tobacco | |
| 14. |
RNA interference helps in |
| A. | Cell proliferation |
| B. | Micropropagation |
| C. | Cell defence |
| D. | Cell differentiation |
| Answer» C. Cell defence | |
| 15. |
Which of the following is the quality of improved transgenic basmati rice? |
| A. | Gives high yield but no characteristic aroma |
| B. | Gives high yield and is rich in vitamin A |
| C. | Does not require chemical fertilizers and growth hormones |
| D. | Resistant to insects and diseases |
| Answer» B. Gives high yield and is rich in vitamin A | |
| 16. |
The first clinical application of gene therapy over a 4 year old girl was for |
| A. | Adenosine deaminase deficiency |
| B. | Adenosine deficiency |
| C. | Growth deficiency |
| D. | Adenine deficiency |
| Answer» A. Adenosine deaminase deficiency | |
| 17. |
Excision and insertion of a gene is called |
| A. | Biotechnology |
| B. | Genetic engineering |
| C. | Cytogenetics |
| D. | Gene therapy |
| Answer» B. Genetic engineering | |
| 18. |
The expression of a transgene in the target tissue is identified by a |
| A. | Transgene |
| B. | Promoter |
| C. | Enhancer |
| D. | Reporter |
| Answer» D. Reporter | |
| 19. |
———– is used as a vector for cloning into higher organisms |
| A. | Retrovirus |
| B. | Baculovirus |
| C. | Salmonella typhimurium |
| D. | Rhizopus nigricans |
| Answer» A. Retrovirus | |
| 20. |
Which bacterium is used in the production of insulin by genetic engineering? |
| A. | Saccharomyces |
| B. | Rhizobium |
| C. | Escherichia |
| D. | Mycobacterium |
| Answer» C. Escherichia | |
| 21. |
The Taq polymerase enzyme is obtained from |
| A. | Thermus aquaticus |
| B. | Thiobacillus ferrooxidans |
| C. | Bacillus subtilis |
| D. | Pseudomonas subtilis |
| Answer» A. Thermus aquaticus | |
| 22. |
Which of the following is an endonuclease? |
| A. | DNase I |
| B. | Hind II |
| C. | Protease |
| D. | RNase restriction |
| Answer» B. Hind II | |
| 23. |
Which of the following restriction enzymes produce blunt ends? |
| A. | Hind III |
| B. | ho |
| C. | Eco RV |
| D. | Sal I |
| Answer» C. Eco RV | |
| 24. |
Which of the following is not a component of downstream processing? |
| A. | Expression |
| B. | Preservation |
| C. | Purification |
| D. | Separation |
| Answer» A. Expression | |
| 25. |
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using |
| A. | Taq polymerase |
| B. | Polymerase III |
| C. | Ligase |
| D. | Eco RI |
| Answer» C. Ligase | |
| 26. |
DNA fragments separated on an agarose gel can be visualized after staining with ____ |
| A. | ethidium bromide |
| B. | bromophenol blue |
| C. | acetocarmine |
| D. | aniline blue |
| Answer» A. ethidium bromide | |
| 27. |
A gene whose expression helps to identify transformed cells is known as ____ |
| A. | Plasmid |
| B. | Selectable marker |
| C. | Structural gene |
| D. | vector |
| Answer» B. Selectable marker | |
| 28. |
A single strand of nucleic acid tagged with a radioactive molecule is called |
| A. | Plasmid |
| B. | Probe |
| C. | selectable marker |
| D. | Vector |
| Answer» B. Probe | |
| 29. |
There is a restriction endonuclease called Eco RI. What does ‘co’ part in it stand for? |
| A. | Coli |
| B. | Colon |
| C. | Cofactor |
| D. | None of the above |
| Answer» A. Coli | |
| 30. |
Agarose extracted from sea weeds finds use in ____ |
| A. | Spectrophotometry |
| B. | Gel electrophoresis |
| C. | PCR |
| D. | tissue culture |
| Answer» B. Gel electrophoresis | |
| 31. |
Animal biotechnology involves |
| A. | production of valuable products in animals using rDNA technology |
| B. | rapid multiplication of animals of desired genotypes |
| C. | alteration of genes to make it more desirable |
| D. | all of these |
| Answer» D. all of these | |
| 32. |
Animal cell cultures are used widely for the production of |
| A. | insulin |
| B. | somatostatin |
| C. | mabs |
| D. | thyroxine |
| Answer» C. mabs | |
| 33. |
The first vaccine developed from animal cell culture was |
| A. | Hepatitis B vaccine |
| B. | Influenza vaccine |
| C. | Small pox vaccine |
| D. | Polio vaccine |
| Answer» D. Polio vaccine | |
| 34. |
Which of the following are commonly produced in animal cell cultures |
| A. | Interferon |
| B. | mab |
| C. | vaccines |
| D. | all of these |
| Answer» D. all of these | |
| 35. |
The cell line used for the production of polio vaccine was |
| A. | Primate kidney cell line |
| B. | CHO cell line |
| C. | Dog kidney cell line |
| D. | mouse fibroblast cell line |
| Answer» A. Primate kidney cell line | |
| 36. |
Recombinant proteins are |
| A. | proteins synthesized in animals |
| B. | proteins synthesized by transgene in host cell by rDNA technology |
| C. | proteins synthesised in cells that are produced by protoplast fusion |
| D. | proteins synthesized in mutated cell lines |
| Answer» B. proteins synthesized by transgene in host cell by rDNA technology | |
| 37. |
Interferons are |
| A. | anti bacterial proteins |
| B. | anti-viral proteins |
| C. | bacteriostatic proteins |
| D. | all of these |
| Answer» B. anti-viral proteins | |
| 38. |
The virus commonly used to infect cell cultures for the production of interferon is |
| A. | Corona virus |
| B. | Sendai virus |
| C. | Polio virus |
| D. | Small pox virus |
| Answer» B. Sendai virus | |
| 39. |
Hybrid antibodies are |
| A. | antibodies produced in cell cultures |
| B. | antibodies designed using rDNA technology produced in cell cultures |
| C. | antibodies produced in invivo |
| D. | both a and b |
| Answer» B. antibodies designed using rDNA technology produced in cell cultures | |
| 40. |
The technique used in animal biotechnology for the rapid multiplication and production of animals with a desirable genotype is |
| A. | protoplast fusion and embryo transfer |
| B. | hybrid selection and embryo transfer |
| C. | in vitro fertilization and embryo transfer |
| D. | all of these |
| Answer» C. in vitro fertilization and embryo transfer | |
| 41. |
The production of complete animals from somatic cells of an animal is called |
| A. | gene cloning |
| B. | animal cloning |
| C. | cell cloning |
| D. | all of these |
| Answer» B. animal cloning | |
| 42. |
The first successfully cloned animal was |
| A. | monkey |
| B. | gibbon |
| C. | sheep |
| D. | rabbit |
| Answer» C. sheep | |
| 43. |
In humans, the babies produced by in vitro fertilization and embryo transfer was popularly called as |
| A. | invitro babies |
| B. | test tube babies |
| C. | invitro-invivo babies |
| D. | all of these |
| Answer» B. test tube babies | |
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