Chapter: Biotechnology and its Applications
451.

Penicillin is obtained from -

A. Mushroom
B. viruses
C. Bacteria and viruses
D. Penicillium notatum
Answer» D. Penicillium notatum
452.

A bioreactor refers to

A. Fermentation tank
B. organisms reacting to stimuli
C. Nuclear reactor for biochemical reactions
D. Tank & biochemical reactions
Answer» D. Tank & biochemical reactions
453.

Cells obtained from cancerous tumours are known as -

A. myeloma
B. hybridomas
C. Lymphocytes
D. Monoclonal cells
Answer» A. myeloma
454.

Hybridomas are employed for

A. synthesis of antibiotic
B. Killing cancer cells
C. synthesis of monoclonal antibodies
D. Production of somatic hybrids
Answer» C. synthesis of monoclonal antibodies
455.

Antibiotics inhibits the growth or destroy

A. Bacteria and fungi
B. Bacteria and viruses
C. Bacteria algae and viruses
D. Bacteria, fungi and viruses
Answer» D. Bacteria, fungi and viruses
456.

Which of the following is not concerned with biotechnology ?

A. Biogas Production
B. Sewage treatment
C. Biofertilizers
D. Wood seasoning
Answer» D. Wood seasoning
Chapter: Biotechnology Principles and Processes
457.

The enzymes that cuts specifically recognition sites in the DNA is known as

A. DNA ligase
B. DNA Polymerase
C. Reverse transcriptase
D. Restriction endonuclease
Answer» D. Restriction endonuclease
458.

DNA can be introduced into any cell by

A. Injection
B. being complexed with Ca salts
C. gel electrophoresis
D. being placed along with
Answer» A. Injection
459.

Ability of a plant or animal cell to repeatedly divide and differentiate into a complete organism is :-

A. cloning
B. DNA finger printing
C. cellular totipotency
D. mitosis
Answer» C. cellular totipotency
460.

Restriction endonuclease is also known as -

A. molecular glue
B. DNA ligase
C. DNA Polymerase
D. molecular scissors
Answer» D. molecular scissors
461.

Extra chromosomal small cirular double stranded DNA molecule in a bacterial cell is stranded DNA molecule in bacterial cell is

A. Plastid
B. Plasmid
C. Mitochondrion
D. Chloroplast
Answer» B. Plasmid
462.

Introduction of foreign genes into plant or animal cells using micropipettes is

A. Electroporation
B. Chemical - mediated genetransfer
C. microinjection
D. Particle gun
Answer» B. Chemical - mediated genetransfer
463.

Which one of the following is releated with genetic engineering ?

A. Mulations
B. Ribosomes
C. Mitochondria
D. Plasmids
Answer» D. Plasmids
464.

In bacteria, genes for antibiotic resistance are usually located in

A. Plasmids
B. Cytoplasm
C. Mitochondria
D. Nucleus
Answer» B. Cytoplasm
465.

A technique used to make numerous copies of a specific segment of DNA quickly and accurately

A. Translation
B. transcription
C. Ligase chain reaction
D. polymerase chain reaction
Answer» B. transcription
466.

The enzyme that cleaves DNA at specific sites, producing sticky ends is called

A. Restriction endonuclease
B. Cleaving enzyme
C. Lysing enzyme
D. Exonuclease
Answer» D. Exonuclease
467.

Which of the Following is a genetic vector ?

A. Plasmid
B. Phage
C. Cosmid
D. All of these
Answer» A. Plasmid
468.

Restriction endonucleases are used in genetic engineering because -

A. They can degrade harmful proteins
B. They can join DNA fragments
C. They can cut DNA at specific base sequences
D. They can cut DNA at variable sites
Answer» D. They can cut DNA at variable sites
469.

Ideal host for the amplification of DNA molecules is

A. Viruses
B. Plants
C. Bacteria
D. Animals
Answer» C. Bacteria
470.

Ti Plasmid naturally occurs in

A. Agro bacterium
B. Corynebacterium
C. Staphylococcus
D. Vibrio
Answer» A. Agro bacterium
471.

The sticky ends of Fragmented DNA molecules are made up of

A. calcuim salts
B. endo nuclease
C. un paired bases
D. methyl groups
Answer» D. methyl groups
472.

Which of the following are the essential requirements for recombination ?

A. Single stranded DNA
B. DNA ligase
C. DNA Polymerase I
D. All of the above
Answer» B. DNA ligase
473.

The Plasmid derived from E.Coli is

A. PBR327
B. PBR322
C. both a above
D. None
Answer» B. PBR322
474.

Ti Plasmid is useful in

A. bringing new genes into animal cells
B. bringing new genes into plant cells
C. to nearly any sites on a chromosome
D. bringing tumour cells into plant cells
Answer» C. to nearly any sites on a chromosome
475.

Many copies of a DNA molecule in a test tube are procurred by

A. Polymerase chain reaction (PCR)
B. Molecular chain reaction (MCR)
C. Ephemeral chain reaction (ECR)
D. All of these
Answer» A. Polymerase chain reaction (PCR)
476.

Bam H I, ECo R I, Sal I are the types of

A. restriction endonucleasses
B. restraction endoxidases
C. restriction exonucleases
D. restriction polymerases
Answer» C. restriction exonucleases
477.

Retro viruses have genetic matetial which is

A. DNA
B. RNA
C. both DAN & RNA
D. proteins
Answer» B. RNA
478.

Genetic engineering is possile because

A. the phenomenon of transducation in bacteria is well understood
B. we can see DNA by electron microscope
C. we can cut DNA at specific sites by endonucleases like DNA ase I
D. restrication endonuclease purified from bacteria can be used in vitro
Answer» A. the phenomenon of transducation in bacteria is well understood
479.

Plasmids are the suitable vectors for genetic cloning as.....

A. they are indispendable
B. they are self replicating units
C. they are essential for bacterial reproducation
D. None of the above
Answer» B. they are self replicating units
480.

Which of the following is used in genetic engineering ?

A. Restrication endonuclease
B. Mycobacterium
C. Entameha
D. Pepsin
Answer» A. Restrication endonuclease
481.

The first hormone artificially produced by culturing bacteria is______

A. Insulin
B. thyroxine
C. Testosterone
D. Adrenaline
Answer» A. Insulin
482.

When the number of genes increases in response to some signal the effect is called.....

A. gene dosage
B. Gene pool
C. gene amplification
D. gene freaquency
Answer» C. gene amplification
483.

Which one of the following pairs is correctly matched ?

A. RNA polymerase - RNA primer
B. Restrication enzymes - Genetic engineering
C. Centeral dogma - codon
D. okazaki fragments - splicing
Answer» B. Restrication enzymes - Genetic engineering
484.

Plasmids are autonomously replicating mini chromosomes found in......

A. Bachterio phage lambda
B. Leishmania donovani
C. Escherichia coli
D. para moecium caudatum
Answer» C. Escherichia coli
485.

Improvement of genotype of an organism by addition of some foreigm gene is......

A. genetic diversity
B. gene handing
C. tissue cutlure
D. genetic engineering
Answer» A. genetic diversity
486.

Two bacteria found to be very useful in genetic engineering experiments are......

A. Nitrosomonas and Klebsiella
B. Escherichia and Agrobacterium
C. Nitrobacter and Azotobacter
D. Rhizobium and Diplococcus
Answer» B. Escherichia and Agrobacterium
487.

Restriction enzymes are isolated chielfy from.......

A. Algae
B. Fungi
C. Protozoans
D. Prokaryotes
Answer» B. Fungi
488.

There are special proteins that help to open up DNA double helix in front of the reaplication work . these proteins are........

A. DNA gyrase
B. DNA polymerase I
C. DNA ligase
D. DNA topoisomerase
Answer» A. DNA gyrase
489.

Technology which uses living components for the welfare of human being is.....

A. Biology
B. Botany
C. Bioinformatics
D. Biotechnology
Answer» D. Biotechnology
490.

Which prosess is involved in making bread cheese, beer and wine ?

A. Respiration / hydrolysis
B. Degradation
C. Fermentation
D. Decomposition
Answer» C. Fermentation
491.

EFB stands for ........

A. European Foudation of Biotechnology
B. European Foundation of Biology
C. European Foundation of Biotechnology
D. European Foundation of Biology
Answer» C. European Foundation of Biotechnology
492.

The organism whoes gene have been artificially altered for desired efect is called as.......

A. genetically mutant organism
B. gene transfer
C. genetically modified organism
D. Genetically transferred organism
Answer» C. genetically modified organism
493.

The sequence of DNA that reads the same backward and forward across the double strand is........

A. Recipient sequence
B. palindromic sequence
C. Replicate sequence
D. origin sequence
Answer» B. palindromic sequence
494.

How many restriction enzymes are known to be isolated ?

A. more than 800
B. more than 700
C. more than 600
D. more than 900
Answer» B. more than 700
495.

Which of the following step is necessary part of DNA recombination technology ?

A. Insertion of DNA fragment into vector
B. Insertion of vector into Bacteria
C. multiplication of the clones containing the recombination molecule
D. All the above
Answer» C. multiplication of the clones containing the recombination molecule
496.

Restriction enzymes belong to which class of enzymes ?

A. Nucleolase
B. Exo nucleases
C. Nucleases
D. Endonucleases
Answer» B. Exo nucleases
497.

A sequence of in a genome at which replication is intiated in .........

A. origin of relpication
B. selectable marker
C. cloning site
D. origin of restriction
Answer» B. selectable marker
498.

Genes which helps in the growth of transformants are .....

A. orgin of replication
B. cloning site
C. origin of restriction
D. selectable marker
Answer» A. orgin of replication
499.

Ti Plasmid is a cloning vector which works with

A. All the plants
B. Dicots only
C. Monocots only
D. Thallophytes only
Answer» B. Dicots only
500.

During which of the following techniques host cells are exposed to pulse of high voltage current ?

A. Electroporation
B. Particle Bombard ments
C. Micro injection
D. lipofection
Answer» A. Electroporation

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