McqMate
These multiple-choice questions (MCQs) are designed to enhance your knowledge and understanding in the following areas: All India Pre Medical Test (AIPMT) .
Chapters
| 501. |
Particle bombardment technique is also known as ..... |
| A. | Lipofection |
| B. | Electroporation |
| C. | Biolistic |
| D. | Micro injection |
| Answer» B. Electroporation | |
| 502. |
Which enzyme is used to break the membrane to relase plant DNA ? |
| A. | Lysozyme |
| B. | Chitinase |
| C. | Cellulose |
| D. | All the above |
| Answer» A. Lysozyme | |
| 503. |
Which enzyne is used to break the membrane to relase animal DNA ? |
| A. | Lysozyme |
| B. | chitinase |
| C. | Celluose |
| D. | All the above |
| Answer» B. chitinase | |
| 504. |
Which is the first step in the process recombinant DNA technology ? |
| A. | denaturing of DNA |
| B. | Annealing of DNA |
| C. | Isolation of Donor DNA |
| D. | Down streaming |
| Answer» A. denaturing of DNA | |
| 505. |
Which primers are used in annealing during amplification of gene ? |
| A. | Reverse primers |
| B. | Forward primers |
| C. | Oligo nucleotide primer |
| D. | Internal primers |
| Answer» A. Reverse primers | |
| 506. |
Which of the following is related with genetic engineering ? |
| A. | Breeding |
| B. | somatic hybridization |
| C. | mutation |
| D. | Transgenic |
| Answer» D. Transgenic | |
| 507. |
What is C - DNA ? |
| A. | circular DNA |
| B. | Cloned DNA |
| C. | DNA produced from reverse transcription of RNA |
| D. | Cytoplasmic DNA |
| Answer» C. DNA produced from reverse transcription of RNA | |
| 508. |
Which of the bollowing statement is incorrect ? |
| A. | cosmid contains gene coding for viral protein |
| B. | cosmid relpicates like plasmids |
| C. | cosmid has antibioticresistant marker gene |
| D. | cos sit has 12 bases helping to join complete genome to make it circular |
| Answer» A. cosmid contains gene coding for viral protein | |
| 509. |
The genetic recombinants obtained by in sertion of plasmid into 1 phage genome is called |
| A. | cosmid |
| B. | plasmid |
| C. | phagmid |
| D. | foreign DNA |
| Answer» A. cosmid | |
| 510. |
TATAATG sequnce near the RNA start point of phokaryotic promoter is...... |
| A. | NICKS |
| B. | DNA marker |
| C. | pallindrome |
| D. | pribnow box |
| Answer» D. pribnow box | |
| 511. |
Exonucleases cleaving nucleotides one at a time from the end of polynucleotide chain are. |
| A. | Specific for 5’ end of RNA strand |
| B. | specific for 3’ end of RNA strand |
| C. | specific for both 5’ and 3’ ends of nucleotide strand |
| D. | Non- specific for 5’ and 3’ ends of nucleotide |
| Answer» B. specific for 3’ end of RNA strand | |
| 512. |
Genes that are involved in turning on or off the transcription of a set of structural genes are called |
| A. | Polymorphic genes |
| B. | operator gene |
| C. | Rebundant gene |
| D. | Regulatory gene |
| Answer» C. Rebundant gene | |
| 513. |
Enzymes used in PCR is .... |
| A. | taq polymerase |
| B. | gyrase |
| C. | transcriptase |
| D. | hexokinase |
| Answer» C. transcriptase | |
| 514. |
What are structure labelled A & B respectively |
| A. | EcoRv restriction endonuclease and E coRv ligase |
| B. | EcoRv ligase and EcoRv nuclease and EcoRvmethlase |
| C. | Eco–– Rv restriction endo EcoRv methylase |
| D. | EcoRv Polymerase and EcoRv methylase |
| Answer» A. EcoRv restriction endonuclease and E coRv ligase | |
| 515. |
Can you pick up from the figure how bacteria protects its own genone from degradation by restreiction endonuclease ? |
| A. | site specific coupling |
| B. | site specific oxidizing |
| C. | site specific oxidizing |
| D. | site specific methylases |
| Answer» D. site specific methylases | |
| 516. |
This is figure of plasmid P BR322 Identiby what represented by A, B, and C |
| A. | A TcR, B ApR and C EcorI |
| B. | A TcR, B EcoRI and C ApR |
| C. | A - EcorI, B ApR and C TcR |
| D. | A ApR, B TcR and C EcoRz |
| Answer» D. A ApR, B TcR and C EcoRz | |
| 517. |
These are important set of enzymes used in biotechnology Match them with exact role
|
| A. | (iii) (iv) (i) (ii) |
| B. | (iii) (v) (iv) (ii) |
| C. | (iv) (i) (v) (ii) |
| D. | (iii) (iv) (i) (ii) |
| Answer» C. (iv) (i) (v) (ii) | |
| 518. |
Match the column I and column II
|
| A. | b a c d e |
| B. | a c b d e |
| C. | a c d b e |
| D. | b c e d a |
| Answer» A. b a c d e | |
| 519. |
For transformation, micro particles coated with DNA are bombarded with gene gun made up of. |
| A. | Platinum or Zinc |
| B. | Silicon or Platinum |
| C. | Gold or tungsten |
| D. | Silver or Platinum |
| Answer» C. Gold or tungsten | |
| 520. |
PCR and Restriction fragment lenth Polymorphism are the methods for. (AIPMT-2012) |
| A. | genetic transtormation |
| B. | DNA Sequencing |
| C. | DNA finger printing |
| D. | Study of enzymes |
| Answer» D. Study of enzymes | |
| 521. |
The linking of antibiotic resistance gene with the plasmid Vector became possible with |
| A. | DNA ligase |
| B. | Exonuclease |
| C. | Endo nuclease |
| D. | DNA Polymerase |
| Answer» C. Endo nuclease | |
| 522. |
Gel electrophoresis is used for-- |
| A. | Isolation of DNA molecule |
| B. | Cutting of DNA in to fragments |
| C. | Separation of DNA fragments according to their size |
| D. | Construction of recombinant DNA by joining with cloning Vector |
| Answer» C. Separation of DNA fragments according to their size | |
| 523. |
Gentic engineering has been sucessfully used for producing ___ (CBSE-2010) |
| A. | trangenic models for studying new treatments for Certain cardiac diseases. |
| B. | transgenic Cow - Rosie which produces high fat milk for making ghee. |
| C. | animals linke bulies for farm work as they have super power. |
| D. | transgenic mice for testing safety of polio Vaccine before use in humans. |
| Answer» D. transgenic mice for testing safety of polio Vaccine before use in humans. | |
| 524. |
Match the following and choose the correct combination from the option given ... (Karnatak PMT-2005)
|
| A. | A = 3, B = 1, C = 5, D = 4 |
| B. | A = 1, B = 2, C = 3, D = 4, |
| C. | A = 2, B = 1, C = 3, D = 4 |
| D. | A = 4, B = 3, C = 1, D = 2 |
| Answer» C. A = 2, B = 1, C = 3, D = 4 | |
| 525. |
Find the incorrrect statement |
| A. | Gene therapy is a genetic engineering technique used to treat disease at molecular level by replacing defective genes with normal genes. |
| B. | Calcitonin is a medically useful recombinant product in the treatment of intetility |
| C. | Bt toxin is a Biodegradable insecticide obtained from bacillis thuringiensis |
| D. | Trichoderma sp. is a biocontrol agent for fungal diseases of plants |
| Answer» A. Gene therapy is a genetic engineering technique used to treat disease at molecular level by replacing defective genes with normal genes. | |
| 526. |
Production of a human protein in bacteria genetic engineering is possible because |
| A. | bacterial cell can carry out the RNA splicing reactions |
| B. | the human chromosome can replicate in bacterial cell |
| C. | the mechanism of gene regulation is identical in human and bacteria |
| D. | The genetic code is universal |
| Answer» C. the mechanism of gene regulation is identical in human and bacteria | |
| 527. |
The basis of DNA finger printing is |
| A. | The double helix |
| B. | Errors in base sequence |
| C. | Poly morphism in sequence |
| D. | DNA replication |
| Answer» D. DNA replication | |
| 528. |
A genetically engineered microorganism used successfully in biomediation of oil spillg is species of (CBSE-2007) |
| A. | Trichoderma |
| B. | Xamthomonas |
| C. | Bacillus |
| D. | Pseudomonas |
| Answer» C. Bacillus | |
| 529. |
What is the function of Restriction endonuclease ? (AIPMT -2006) |
| A. | Restricts the synthesis of DNA inside the nucleus |
| B. | Synthesizes DNA |
| C. | Cuts DNA molecule randomly |
| D. | cuts DNA molecule at specific sites |
| Answer» D. cuts DNA molecule at specific sites | |
| 530. |
The nuclease enzyme which begins its attack from Free end of a polynucleotide is |
| A. | Exonuclease |
| B. | Kinase |
| C. | Polymerase |
| D. | Endonuclease |
| Answer» A. Exonuclease | |
| 531. |
Identify the Plasmid (ET 2004) |
| A. | Alu I |
| B. | Hind III |
| C. | ECORI |
| D. | PBR322 |
| Answer» C. ECORI | |
| 532. |
Molecular scissors, which cut DNA at specific site (Kerala-2004) |
| A. | ligase |
| B. | cellulase |
| C. | pectinase |
| D. | Polymerase |
| Answer» E. | |
| 533. |
In transgenics the experession of transce in the target tissue is known by (CBSE-2004) |
| A. | Enhancer |
| B. | Transgene |
| C. | Promoter |
| D. | Reporter |
| Answer» A. Enhancer | |
| 534. |
Variable number of tender repeats (VTNR) in the DNA molecule are highly useful in |
| A. | monoclonal antibody production |
| B. | DNA finger printing |
| C. | Recombinant DNA technology |
| D. | stem cell culture |
| Answer» B. DNA finger printing | |
| 535. |
Which one of the following bacteria has found extensive use in genetic engineering work in plants ? |
| A. | Agrobacterium tamefaciens |
| B. | Clostridium septicum |
| C. | Xanthomonas citri |
| D. | Bacilius Coagulens |
| Answer» A. Agrobacterium tamefaciens | |
| 536. |
What does Bt stand For the Popular crop Bt Cotton ? |
| A. | Best |
| B. | Best type |
| C. | Biotechnology |
| D. | Bacilius tomentosta |
| Answer» E. | |
| 537. |
The total number of nitrogenous bases in human genome is estimated to be about |
| A. | 35 million |
| B. | 3.1 million |
| C. | 3.5 million |
| D. | 3.5 thousand |
| Answer» D. 3.5 thousand | |
| 538. |
Name of the drug used in cancer treatment produced by using biotechnology is ..... |
| A. | HGH |
| B. | TSH |
| C. | Insulin |
| D. | Interfern |
| Answer» C. Insulin | |
| 539. |
Which of the following pair is correctly matched ? |
| A. | - central dogma - codon |
| B. | - Okazaki fragments - splicing |
| C. | RNA Polymerase - RNA Primer |
| D. | Restriction enzymes - genetic engineering |
| Answer» C. RNA Polymerase - RNA Primer | |
| 540. |
First Biochemical to be Producod commer cially by microbial cloning and genetic engineering is ___ |
| A. | interferom |
| B. | penicillin |
| C. | human insulin |
| D. | Fertility factors |
| Answer» A. interferom | |
| 541. |
First hormone prepared by genetic engineering is .... |
| A. | Insulin |
| B. | Oxytocin |
| C. | adrenaline |
| D. | Somatotropin |
| Answer» A. Insulin | |
| 542. |
A technology which has found immense use in solving cases of disputed parentage is |
| A. | DNA finger printing |
| B. | Polymerase chain reaction |
| C. | Recombinant DNA technology |
| D. | Monoclonal antibody production |
| Answer» A. DNA finger printing | |
| 543. |
Matching sequence of DNA between two evidences, one of the criminal with the suspect is known as |
| A. | DNA finger printing |
| B. | DNA amplification |
| C. | Gene maping |
| D. | DNA resolution |
| Answer» D. DNA resolution | |
| 544. |
Agarose extracted From weeds finds use in ______ (A.I.PMT 2011) |
| A. | spectrophoto metry |
| B. | Tissue culture |
| C. | Gel electrophoresis |
| D. | PCR |
| Answer» B. Tissue culture | |
| 545. |
Widely used tool in genetic engineering of crop plants is ____ (AIEEE 2004) |
| A. | protoplast fusion |
| B. | Transposon |
| C. | Micro injection |
| D. | Agrobacterium mediation |
| Answer» B. Transposon | |
| 546. |
c DNA Probes are copied from messenger RNA molecule with the help of __ |
| A. | Restriction enzyme |
| B. | Reverse transcriptase |
| C. | DNA Polymerase |
| D. | Adenosine deaminase |
| Answer» D. Adenosine deaminase | |
| 547. |
Which one of the following pair is wrongly matched ? |
| A. | methanogens - Gobargas |
| B. | Yeast - Ethanol |
| C. | Streptomycetes - Antibiotic |
| D. | Coliborms - vinegar |
| Answer» D. Coliborms - vinegar | |
| 548. |
The Prerequisites for biotechnological production of antibiotic is |
| A. | to search an antibiotic producing microorganism |
| B. | to isolate the antibiotic gene |
| C. | to join antibiotic gene with E coli plasmid |
| D. | All of the above (MP PMT 2008) |
| Answer» D. All of the above (MP PMT 2008) | |
| 549. |
Which one of the following is now being commercially produced by biotechnological Procedures |
| A. | Nicotine |
| B. | Morphine |
| C. | quinine |
| D. | Insulin |
| Answer» D. Insulin | |
| 550. |
Which one of the following is a wrong matching of a microbe and its industrial product while the remaining three are correct |
| A. | clostridium butylicum - lactic acid |
| B. | Aspergillis niger cirric acid |
| C. | yeast - statins |
| D. | Acetobacter aceti - acetic acid (CBSE PMT 2011) |
| Answer» A. clostridium butylicum - lactic acid | |
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