McqMate
These multiple-choice questions (MCQs) are designed to enhance your knowledge and understanding in the following areas: All India Pre Medical Test (AIPMT) .
Chapters
| 1. |
The enzymes that cuts specifically recognition sites in the DNA is known as |
| A. | DNA ligase |
| B. | DNA Polymerase |
| C. | Reverse transcriptase |
| D. | Restriction endonuclease |
| Answer» D. Restriction endonuclease | |
| 2. |
DNA can be introduced into any cell by |
| A. | Injection |
| B. | being complexed with Ca salts |
| C. | gel electrophoresis |
| D. | being placed along with |
| Answer» A. Injection | |
| 3. |
Ability of a plant or animal cell to repeatedly divide and differentiate into a complete organism is :- |
| A. | cloning |
| B. | DNA finger printing |
| C. | cellular totipotency |
| D. | mitosis |
| Answer» C. cellular totipotency | |
| 4. |
Restriction endonuclease is also known as - |
| A. | molecular glue |
| B. | DNA ligase |
| C. | DNA Polymerase |
| D. | molecular scissors |
| Answer» D. molecular scissors | |
| 5. |
Extra chromosomal small cirular double stranded DNA molecule in a bacterial cell is stranded DNA molecule in bacterial cell is |
| A. | Plastid |
| B. | Plasmid |
| C. | Mitochondrion |
| D. | Chloroplast |
| Answer» B. Plasmid | |
| 6. |
Introduction of foreign genes into plant or animal cells using micropipettes is |
| A. | Electroporation |
| B. | Chemical - mediated genetransfer |
| C. | microinjection |
| D. | Particle gun |
| Answer» B. Chemical - mediated genetransfer | |
| 7. |
Which one of the following is releated with genetic engineering ? |
| A. | Mulations |
| B. | Ribosomes |
| C. | Mitochondria |
| D. | Plasmids |
| Answer» D. Plasmids | |
| 8. |
In bacteria, genes for antibiotic resistance are usually located in |
| A. | Plasmids |
| B. | Cytoplasm |
| C. | Mitochondria |
| D. | Nucleus |
| Answer» B. Cytoplasm | |
| 9. |
A technique used to make numerous copies of a specific segment of DNA quickly and accurately |
| A. | Translation |
| B. | transcription |
| C. | Ligase chain reaction |
| D. | polymerase chain reaction |
| Answer» B. transcription | |
| 10. |
The enzyme that cleaves DNA at specific sites, producing sticky ends is called |
| A. | Restriction endonuclease |
| B. | Cleaving enzyme |
| C. | Lysing enzyme |
| D. | Exonuclease |
| Answer» D. Exonuclease | |
| 11. |
Which of the Following is a genetic vector ? |
| A. | Plasmid |
| B. | Phage |
| C. | Cosmid |
| D. | All of these |
| Answer» A. Plasmid | |
| 12. |
Restriction endonucleases are used in genetic engineering because - |
| A. | They can degrade harmful proteins |
| B. | They can join DNA fragments |
| C. | They can cut DNA at specific base sequences |
| D. | They can cut DNA at variable sites |
| Answer» D. They can cut DNA at variable sites | |
| 13. |
Ideal host for the amplification of DNA molecules is |
| A. | Viruses |
| B. | Plants |
| C. | Bacteria |
| D. | Animals |
| Answer» C. Bacteria | |
| 14. |
Ti Plasmid naturally occurs in |
| A. | Agro bacterium |
| B. | Corynebacterium |
| C. | Staphylococcus |
| D. | Vibrio |
| Answer» A. Agro bacterium | |
| 15. |
The sticky ends of Fragmented DNA molecules are made up of |
| A. | calcuim salts |
| B. | endo nuclease |
| C. | un paired bases |
| D. | methyl groups |
| Answer» D. methyl groups | |
| 16. |
Which of the following are the essential requirements for recombination ? |
| A. | Single stranded DNA |
| B. | DNA ligase |
| C. | DNA Polymerase I |
| D. | All of the above |
| Answer» B. DNA ligase | |
| 17. |
The Plasmid derived from E.Coli is |
| A. | PBR327 |
| B. | PBR322 |
| C. | both a above |
| D. | None |
| Answer» B. PBR322 | |
| 18. |
Ti Plasmid is useful in |
| A. | bringing new genes into animal cells |
| B. | bringing new genes into plant cells |
| C. | to nearly any sites on a chromosome |
| D. | bringing tumour cells into plant cells |
| Answer» C. to nearly any sites on a chromosome | |
| 19. |
Many copies of a DNA molecule in a test tube are procurred by |
| A. | Polymerase chain reaction (PCR) |
| B. | Molecular chain reaction (MCR) |
| C. | Ephemeral chain reaction (ECR) |
| D. | All of these |
| Answer» A. Polymerase chain reaction (PCR) | |
| 20. |
Bam H I, ECo R I, Sal I are the types of |
| A. | restriction endonucleasses |
| B. | restraction endoxidases |
| C. | restriction exonucleases |
| D. | restriction polymerases |
| Answer» C. restriction exonucleases | |
| 21. |
Retro viruses have genetic matetial which is |
| A. | DNA |
| B. | RNA |
| C. | both DAN & RNA |
| D. | proteins |
| Answer» B. RNA | |
| 22. |
Genetic engineering is possile because |
| A. | the phenomenon of transducation in bacteria is well understood |
| B. | we can see DNA by electron microscope |
| C. | we can cut DNA at specific sites by endonucleases like DNA ase I |
| D. | restrication endonuclease purified from bacteria can be used in vitro |
| Answer» A. the phenomenon of transducation in bacteria is well understood | |
| 23. |
Plasmids are the suitable vectors for genetic cloning as..... |
| A. | they are indispendable |
| B. | they are self replicating units |
| C. | they are essential for bacterial reproducation |
| D. | None of the above |
| Answer» B. they are self replicating units | |
| 24. |
Which of the following is used in genetic engineering ? |
| A. | Restrication endonuclease |
| B. | Mycobacterium |
| C. | Entameha |
| D. | Pepsin |
| Answer» A. Restrication endonuclease | |
| 25. |
The first hormone artificially produced by culturing bacteria is______ |
| A. | Insulin |
| B. | thyroxine |
| C. | Testosterone |
| D. | Adrenaline |
| Answer» A. Insulin | |
| 26. |
When the number of genes increases in response to some signal the effect is called..... |
| A. | gene dosage |
| B. | Gene pool |
| C. | gene amplification |
| D. | gene freaquency |
| Answer» C. gene amplification | |
| 27. |
Which one of the following pairs is correctly matched ? |
| A. | RNA polymerase - RNA primer |
| B. | Restrication enzymes - Genetic engineering |
| C. | Centeral dogma - codon |
| D. | okazaki fragments - splicing |
| Answer» B. Restrication enzymes - Genetic engineering | |
| 28. |
Plasmids are autonomously replicating mini chromosomes found in...... |
| A. | Bachterio phage lambda |
| B. | Leishmania donovani |
| C. | Escherichia coli |
| D. | para moecium caudatum |
| Answer» C. Escherichia coli | |
| 29. |
Improvement of genotype of an organism by addition of some foreigm gene is...... |
| A. | genetic diversity |
| B. | gene handing |
| C. | tissue cutlure |
| D. | genetic engineering |
| Answer» A. genetic diversity | |
| 30. |
Two bacteria found to be very useful in genetic engineering experiments are...... |
| A. | Nitrosomonas and Klebsiella |
| B. | Escherichia and Agrobacterium |
| C. | Nitrobacter and Azotobacter |
| D. | Rhizobium and Diplococcus |
| Answer» B. Escherichia and Agrobacterium | |
| 31. |
Restriction enzymes are isolated chielfy from....... |
| A. | Algae |
| B. | Fungi |
| C. | Protozoans |
| D. | Prokaryotes |
| Answer» B. Fungi | |
| 32. |
There are special proteins that help to open up DNA double helix in front of the reaplication work . these proteins are........ |
| A. | DNA gyrase |
| B. | DNA polymerase I |
| C. | DNA ligase |
| D. | DNA topoisomerase |
| Answer» A. DNA gyrase | |
| 33. |
Technology which uses living components for the welfare of human being is..... |
| A. | Biology |
| B. | Botany |
| C. | Bioinformatics |
| D. | Biotechnology |
| Answer» D. Biotechnology | |
| 34. |
Which prosess is involved in making bread cheese, beer and wine ? |
| A. | Respiration / hydrolysis |
| B. | Degradation |
| C. | Fermentation |
| D. | Decomposition |
| Answer» C. Fermentation | |
| 35. |
EFB stands for ........ |
| A. | European Foudation of Biotechnology |
| B. | European Foundation of Biology |
| C. | European Foundation of Biotechnology |
| D. | European Foundation of Biology |
| Answer» C. European Foundation of Biotechnology | |
| 36. |
The organism whoes gene have been artificially altered for desired efect is called as....... |
| A. | genetically mutant organism |
| B. | gene transfer |
| C. | genetically modified organism |
| D. | Genetically transferred organism |
| Answer» C. genetically modified organism | |
| 37. |
The sequence of DNA that reads the same backward and forward across the double strand is........ |
| A. | Recipient sequence |
| B. | palindromic sequence |
| C. | Replicate sequence |
| D. | origin sequence |
| Answer» B. palindromic sequence | |
| 38. |
How many restriction enzymes are known to be isolated ? |
| A. | more than 800 |
| B. | more than 700 |
| C. | more than 600 |
| D. | more than 900 |
| Answer» B. more than 700 | |
| 39. |
Which of the following step is necessary part of DNA recombination technology ? |
| A. | Insertion of DNA fragment into vector |
| B. | Insertion of vector into Bacteria |
| C. | multiplication of the clones containing the recombination molecule |
| D. | All the above |
| Answer» C. multiplication of the clones containing the recombination molecule | |
| 40. |
Restriction enzymes belong to which class of enzymes ? |
| A. | Nucleolase |
| B. | Exo nucleases |
| C. | Nucleases |
| D. | Endonucleases |
| Answer» B. Exo nucleases | |
| 41. |
A sequence of in a genome at which replication is intiated in ......... |
| A. | origin of relpication |
| B. | selectable marker |
| C. | cloning site |
| D. | origin of restriction |
| Answer» B. selectable marker | |
| 42. |
Genes which helps in the growth of transformants are ..... |
| A. | orgin of replication |
| B. | cloning site |
| C. | origin of restriction |
| D. | selectable marker |
| Answer» A. orgin of replication | |
| 43. |
Ti Plasmid is a cloning vector which works with |
| A. | All the plants |
| B. | Dicots only |
| C. | Monocots only |
| D. | Thallophytes only |
| Answer» B. Dicots only | |
| 44. |
During which of the following techniques host cells are exposed to pulse of high voltage current ? |
| A. | Electroporation |
| B. | Particle Bombard ments |
| C. | Micro injection |
| D. | lipofection |
| Answer» A. Electroporation | |
| 45. |
Particle bombardment technique is also known as ..... |
| A. | Lipofection |
| B. | Electroporation |
| C. | Biolistic |
| D. | Micro injection |
| Answer» B. Electroporation | |
| 46. |
Which enzyme is used to break the membrane to relase plant DNA ? |
| A. | Lysozyme |
| B. | Chitinase |
| C. | Cellulose |
| D. | All the above |
| Answer» A. Lysozyme | |
| 47. |
Which enzyne is used to break the membrane to relase animal DNA ? |
| A. | Lysozyme |
| B. | chitinase |
| C. | Celluose |
| D. | All the above |
| Answer» B. chitinase | |
| 48. |
Which is the first step in the process recombinant DNA technology ? |
| A. | denaturing of DNA |
| B. | Annealing of DNA |
| C. | Isolation of Donor DNA |
| D. | Down streaming |
| Answer» A. denaturing of DNA | |
| 49. |
Which primers are used in annealing during amplification of gene ? |
| A. | Reverse primers |
| B. | Forward primers |
| C. | Oligo nucleotide primer |
| D. | Internal primers |
| Answer» A. Reverse primers | |
| 50. |
Which of the following is related with genetic engineering ? |
| A. | Breeding |
| B. | somatic hybridization |
| C. | mutation |
| D. | Transgenic |
| Answer» D. Transgenic | |
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