Chapter: Biotechnology and its Applications
1.

In which of the following industrial areas biotechnology is applicable?

A. Health care
B. Environment
C. Agriculture
D. all of the above
Answer» D. all of the above
2.

Due to what food supply has increased during green revolution?

A. use of chemical
B. use of biochemicals
C. use of photochemicals
D. use of agrochemicals
Answer» D. use of agrochemicals
3.

The organizations whose genetic material has been altered using genetic engineering is called as -

A. Genetically mutant organism
B. Genetically modern organism
C. Genetically modified organism
D. Genetically transferred organism.
Answer» C. Genetically modified organism
4.

Full form of GMO is -

A. Genetically mutant organism
B. Genetically modern organism
C. Genetically modified organism
D. Genetically transferred organism
Answer» C. Genetically modified organism
5.

Which of the following is responsible for causing disease in plants?

A. viru
B. pesticide
C. Herbicide
D. all the above
Answer» A. viru
6.

Bt stands for -

A. Biotechnology
B. Bacteria tolerant
C. Bacillus thuringiensis
D. Bollworm toxin
Answer» C. Bacillus thuringiensis
7.

Where from Bt toxin gene has been obtained?

A. plant
B. virus
C. Bacteria
D. Fungi
Answer» C. Bacteria
8.

In which of the following plant Bt toxin gene is expressed?

A. Bt cotton
B. Bt corn
C. Bt Bringal
D. All the above
Answer» A. Bt cotton
9.

Toxin proteins produced by the bacterial gene destroys -

A. Aphid
B. grass hoppers
C. Boll worms
D. Beetles
Answer» A. Aphid
10.

The toxin produced by the Bt is coded by a gene named as -

A. Cry
B. Cry Protein
C. Cyr
D. Cyr Protein
Answer» B. Cry Protein
11.

Which of the following techniques use genes and DNA molecules for diagnoses of diseases?

A. Gene therapy
B. Recombinant gene technology
C. Polymerase chain reaction
D. All the above
Answer» D. All the above
12.

What were the earlier sources of Insulin?

A. cattle and pig
B. camel and pig
C. cattle and dog
D. goat and pig
Answer» A. cattle and pig
13.

Which technology was used to produce human Insulin in E. coli ?

A. Gene therapy
B. Recombinant gene technology
C. Polymerase chain reaction
D. All the above
Answer» D. All the above
14.

Which therapy is used for modification of germ cells (sperms and eggs)?

A. sperm line therapy
B. Germ line therapy
C. Egg line therapy
D. Germ line gene therapy
Answer» D. Germ line gene therapy
15.

Modifications by germ line gene therapy are heritable as -

A. The Functional gene is incorporated into to their genome.
B. The Functional gene is incorporated into one of the gene.
C. The Functional gene is incorporated into somatic cells.
D. All the above
Answer» A. The Functional gene is incorporated into to their genome.
16.

In which therapy, vectors are used to introduce desired gene into the body of patients?

A. In vivo, gene therapy
B. Germ line therapy
C. Ex vivo gene therapy
D. Foreign gene therapy
Answer» A. In vivo, gene therapy
17.

The method during which genetic characteristics of animals are improved by mating of selected breeds is known as -

A. Improved breeding
B. selective breeding
C. mating
D. Breeding
Answer» B. selective breeding
18.

The Phenomenon of introduction of exogenous DNA into the genome of animals is -

A. In vivo, gene therapy
B. Foreign gene therapy
C. Ex vivo gene therapy
D. Transgenesis
Answer» D. Transgenesis
19.

The animals whose genome is altered by introduction of transgene is known as -

A. modified animal
B. Hybrid animals
C. cross breed animals
D. Transgenic animal
Answer» D. Transgenic animal
20.

Transgene introduced in the first transgenic cow was responsible for the production of

A. Albumin enriched milk
B. Protein enriched milk
C. Human protein enriched milk
D. Vitamins enriched milk
Answer» C. Human protein enriched milk
21.

What was the amount of Alpha-lactalbumin in the milk of transgenic cow ?

A. 4.2 grams per litre
B. 2.4 grams per litre
C. 3.4 grams per litre
D. 4.2 grams per litre
Answer» B. 2.4 grams per litre
22.

Earlier which animals were used to test the safety of Polio Vaccine

A. Transgenic rat
B. Transgenic pigs
C. Transgenic mice
D. Transgenic sheep
Answer» C. Transgenic mice
23.

The right granted by government to prevent others from the commercial use of resources invention is -

A. Government grant
B. Patent
C. official document
D. Biopatent
Answer» D. Biopatent
24.

Exploitation of Patent of biological resources of other nations is called as -

A. Biosafety
B. Biopiracy
C. Biowar
D. Bioabuse
Answer» B. Biopiracy
25.

Organisms which can be used to gain commercial benefits are called -

A. Beneficial resource
B. Bioresources
C. Financial resources
D. Biological resources
Answer» B. Bioresources
26.

The plant Pentadiplandra brazzeana belongs to which country ?

A. China
B. West Africa
C. Pakistan
D. America
Answer» B. West Africa
27.

Which technology facilitates the production of novel DNA molecule by combining sequences from DNA from two different organisms ?

A. gene therapy
B. Recombinant DNA technology
C. Polymerase chain reaction
D. germ line gene therapy
Answer» B. Recombinant DNA technology
28.

Which is the most common bioinsecticide for the protection of cotton ?

A. Pyrethrin
B. Rotenone
C. Eicer
D. Bacillus thuringiensis
Answer» D. Bacillus thuringiensis
29.

Which of the following can be controlled with the help of biopesticides ?

A. Insect
B. Disease
C. Weeds
D. All the above
Answer» D. All the above
30.

Transgenic animals are produced by incorporation of Foreign gene into the -

A. Nucleus of fertilized egg
B. Nucleus of sperm
C. Nucleus of unfertilized egg
D. Egg cell
Answer» A. Nucleus of fertilized egg
31.

The bacteria associated with plant genetic engineering are -

A. salmonella and Pseudomonas
B. Salmonella typhimurium and agrobacterium
C. Bacillus thuringiensis and Pseudomonas fluorescens
D. Both b and c
Answer» D. Both b and c
32.

The science of biotechnology has contributed to field of

A. Health
B. Pharmacy
C. agriculture and industry
D. all above
Answer» D. all above
33.

The method of producing proteins for food or feed through microbial biomass is called

A. PCR
B. SCP
C. Nanotechnology
D. None of above
Answer» B. SCP
34.

The insulin prepared through genetic engineering is called

A. Human insulin
B. microbial insulin
C. Bio insulin
D. Humulin
Answer» D. Humulin
35.

The most common Bioinsecticide in present in the world for protection and mustard is——

A. Pyrethrin
B. Bt. ,
C. Rotenone
D. none of these
Answer» B. Bt. ,
36.

First progress in field of genetic engineering, in 1978 by Cohen, Berg and Boyer by synthesis of— ————through E. coli

A. Insulin
B. growth hormon
C. Somatostatin
D. both b and c
Answer» D. both b and c
37.

Which was the first product contributed to human kind by science of Biotechnology in early 1972

A. somatotropin
B. Insulin
C. cytokines
D. erythropoietin
Answer» A. somatotropin
38.

Which is the most common Plasmid used for most of genetically engineered product ?

A. PBR322
B. R Plasmid types
C. CaMV195
D. both a and b
Answer» D. both a and b
39.

The main use of recombinant DNA technology are.....

A. production of transgenic humans.
B. the creation of cells capable of synthesizing economically important molecules.
C. the efficient reduction of useful proteins.
D. both b and c
Answer» D. both b and c
40.

Biofuel is made by utilizing which strain of bacteria ?

A. Bacillus amyloliquefacien
B. Klebsiella Planticola
C. E. coli
D. Phanerochaete chrysosporium
Answer» A. Bacillus amyloliquefacien
41.

Bio augmentation is …..

A. the addition of commercially prepared bacterial strain
B. Production of fertilizers by using bacteria
C. the metals are deposited as insoluble oxides and sulphides by activities of bacteria
D. removal of pests
Answer» A. the addition of commercially prepared bacterial strain
42.

Which of the following animal is best known genetically ?

A. Planaria
B. Domestic dog
C. Musca domestica
D. Drosophila melanogaster
Answer» D. Drosophila melanogaster
43.

Which one is a transgenic crop ?

A. Brinjal
B. Potato
C. Grape
D. Tomato
Answer» D. Tomato
44.

First transgenic mouse grew twice the normal size after drinking _______ containing water.

A. cu
B. Fe
C. Zn
D. Ra
Answer» C. Zn
45.

The genetically modified crops introduced in India are …

A. cotton
B. mustard
C. Wild plant
D. Both a and b
Answer» D. Both a and b
46.

One of the following is the correct sequence to make a transgenic animals.

A. Transomics – transfection – micro infection – electro portion – retroviral vectors
B. Micro injection - transfection - electro portion - retroviral vectors - transomics
C. Transfection – micro injection - transomics - electro portion - retroviral vectors
D. None of these
Answer» B. Micro injection - transfection - electro portion - retroviral vectors - transomics
47.

one of the following is transgenic organisms

A. Holly sheep and tomato
B. Dolly sheep and subabul
C. Molly sheep and banana
D. B T cotton and tomato (Flaur saur)
Answer» D. B T cotton and tomato (Flaur saur)
48.

Transgenic plants are produced by using Ti Plasmids from the

A. Agrobacterium tumefacien
B. E. coli
C. Bacteriophage
D. Agrobacterium varians
Answer» A. Agrobacterium tumefacien
49.

Vaccine is a …...

A. collection of antibiotics
B. collection of life saving drugs
C. collection of killed disease causing bacteria and virus
D. collection of lysins
Answer» C. collection of killed disease causing bacteria and virus
50.

The typical machine for production of bio-products through microbial is

A. sterilized glass ware
B. microprojectile
C. autoclave
D. Fermenter
Answer» D. Fermenter
51.

The foods made from genetically modified crops required to Pass human testing because

A. they may cause allergie
B. they may alter genes
C. they may cause mutations and release toxins
D. all above
Answer» D. all above
52.

There are set of health care products. Match them with organisms which are genetically engineered for respective products.
A. Insulin 1. Escherichia coli / saccharomyces
B. Somatotropin 2. Escherichia coli / yeast
C. Interferon 3. G M E coli
D. Interleukins 4. hGH in E. coli
5. Humulin through E. coli
A B C D

A. 5 4 1 2
B. 5 1 1 4
C. 5 3 4 1
D. 5 4 3 2
Answer» A. 5 4 1 2
53.

Pick up the correct set.
A. Spirulina 1. Non surfacetant polymers for oil recovery
B. Monilia / Fusarium 2. SCP
C. Methanobrevibacter 3. Ethanol from carbohydrates
D. Aureobasidium 4. Curd formation
5. Biogas formation
A B C D

A. 2 3 5 1
B. 2 5 4 1
C. 2 4 3 5
D. 2 1 4 5
Answer» A. 2 3 5 1
54.

Tissue culture technique has been biotechnologically successful in production of -

A. alcoholic beverage
B. cheese
C. shikonin
D. Insulin
Answer» C. shikonin
55.

Yeast is a good source of

A. carbohydrate
B. vitamin B
C. Proteins
D. both b and c
Answer» C. Proteins
56.

The micro – organism involves in making bread is …..

A. Acetobacter
B. brewer’s yeast
C. Saccharomyces cerevisiae
D. None of the above
Answer» C. Saccharomyces cerevisiae
57.

Penicillin is obtained from -

A. Mushroom
B. viruses
C. Bacteria and viruses
D. Penicillium notatum
Answer» D. Penicillium notatum
58.

A bioreactor refers to

A. Fermentation tank
B. organisms reacting to stimuli
C. Nuclear reactor for biochemical reactions
D. Tank & biochemical reactions
Answer» D. Tank & biochemical reactions
59.

Cells obtained from cancerous tumours are known as -

A. myeloma
B. hybridomas
C. Lymphocytes
D. Monoclonal cells
Answer» A. myeloma
60.

Hybridomas are employed for

A. synthesis of antibiotic
B. Killing cancer cells
C. synthesis of monoclonal antibodies
D. Production of somatic hybrids
Answer» C. synthesis of monoclonal antibodies
61.

Antibiotics inhibits the growth or destroy

A. Bacteria and fungi
B. Bacteria and viruses
C. Bacteria algae and viruses
D. Bacteria, fungi and viruses
Answer» D. Bacteria, fungi and viruses
62.

Which of the following is not concerned with biotechnology ?

A. Biogas Production
B. Sewage treatment
C. Biofertilizers
D. Wood seasoning
Answer» D. Wood seasoning
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