Chapter: Molecular Basis of Inheritance
1.

Name the conjugated protein used as genetic material in living cells

A. Glyco protein
B. Nucleo protein
C. Metallo protein
D. Lip oprotein
Answer» B. Nucleo protein
2.

Who supported Griffith effect by molecular explanation ?

A. Hershay and chase
B. Watson, crick Ninenberg
C. Avery , Mc Carty and Macleod
D. Griffith and Avery
Answer» C. Avery , Mc Carty and Macleod
3.

Synthesis of nucleic acids always takes place in

A. 31 - 51 dicectioon
B. 51 - 31 direction
C. Both ways
D. in any direction
Answer» B. 51 - 31 direction
4.

What is called Griffith effect ?

A. DNA transcription
B. RNA translation
C. Bacterial transformation
D. Bacterial transduction
Answer» C. Bacterial transformation
5.

Genetic information is carried by the long chain molecules which are made up of

A. Amino acids
B. Nucleotides
C. Chromosomes
D. Enzymes
Answer» B. Nucleotides
6.

By which bonds the purine & pyrimidine pairs of Complementary Strands of DNA held together?

A. H - bonds
B. O - bonds
C. C - bonds
D. N - bonds
Answer» A. H - bonds
7.

State the nature of the 2 Strands of DNA duplex.

A. identical & Complementary
B. Anti parallel & complementary
C. Disimilar & non - complementary
D. Anti parallel & Non - complementary
Answer» B. Anti parallel & complementary
8.

The code AUG stands for

A. Glycine
B. Methionine
C. N-formyl methionine
D. A lanine
Answer» B. Methionine
9.

A Codon is made up of

A. Single nucleotide
B. two nucleotides
C. three nucleotides
D. Four nucleotides
Answer» C. three nucleotides
10.

Nucleus of a cell is the site of Synthesis of

A. DNA
B. m - RNA
C. t - RNA
D. All
Answer» D. All
11.

DNA replication requires

A. DNA polymerase only
B. DNA polymerase and ligase
C. Ligase only
D. RNA polymerase
Answer» B. DNA polymerase and ligase
12.

The enzyme involved in transcription is

A. RNA polymerase
B. DNA polymerase I
C. DNA polymerase II
D. DNA polymerase III
Answer» A. RNA polymerase
13.

Enzymes needed for formation of repliction fork

A. RNA polymerase and DNA polymerase I
B. Helicase and gyrase
C. Hexokinase and aldolase
D. Ligase and endo nuclease
Answer» B. Helicase and gyrase
14.

Okazaki fragments are Synthesized on

A. Leading strands of DNA only
B. Lagging Strands of DNA only
C. Leading and LaggingStrands
D. Complementary DNA Strand
Answer» B. Lagging Strands of DNA only
15.

Which of the following is used in DNA multiplication?

A. RNA polymerase
B. DNA endonuclease
C. DNA exonuclease
D. DNA Polymerase
Answer» D. DNA Polymerase
16.

t - RNA attaches aminoacid at its

A. 31 end
B. 51 end
C. Anticodon
D. Loop
Answer» A. 31 end
17.

DNA acts as a template for synthesis of

A. RNA
B. DNA
C. Both ‘a’ and ‘b’
D. Protein
Answer» C. Both ‘a’ and ‘b’
18.

Antiparallel strand in DNA is due to

A. Disulphide linkage
B. Hydorgen bond
C. Phosphodiester bond
D. Ionic bond
Answer» C. Phosphodiester bond
19.

Multiplication of DNA is called

A. Translation
B. Replication
C. Transduction
D. Transcription
Answer» B. Replication
20.

Which is the smallest RNA ?

A. r RNA
B. m RNA
C. t - RNA
D. nuclear RNA
Answer» C. t - RNA
21.

Genetic information are transfered from nucleus to cytoplasm of cell through

A. DNA
B. RNA
C. Lysosomes
D. Anticodon
Answer» B. RNA
22.

The information from RNA to DNA are transfered by which process

A. Replication
B. Transcription
C. Translation
D. Reverse transcription
Answer» D. Reverse transcription
23.

Which statement is correct ?
(A) Degeneracy of code is related to third member of codon
(B) Single codon, codes for more than one aminoacid
(C) In codon first two bases are more specific
(D) In codons third base is wobble
(E) code is universal

A. A,B,C,D,E
B. A, B, D
C. A., C, D
D. A, C, D, E
Answer» D. A, C, D, E
24.

DNA molecule has uniform diameter due to ?

A. Double stranded
B. Presence of phosphate
C. Specific base pairing between purine and pyrimidine
D. Specific base pairing between purine and purine
Answer» C. Specific base pairing between purine and pyrimidine
25.

In a transcription unit promotor is said to be located towards

A. 31 end of structural gene
B. 51 end of structural gene
C. 51 end of template strand
D. 31 end of template strand
Answer» D. 31 end of template strand
26.

In DNA replication the primer is

A. A Small deoxyribonucleotide polymer
B. A small ribonucleotide polymer
C. Helix destalilizing protein
D. Enzyme taking part in joining nucleotides of new strands
Answer» B. A small ribonucleotide polymer
27.

Non - sense codons take part in

A. formation of unspecified aminoacids
B. Terminating message of gene controlled protein synthesis
C. Releasing t-RNA from polynucleotide chain
D. Conversion of sense DNA in to non-sense one
Answer» B. Terminating message of gene controlled protein synthesis
28.

select the correct sequence of following in DNA replication

A. single stranded binding proteins - Helicase - Topoisomerase - DNA polymerase
B. Helicase - single stranded binding proteins -Topoisomerase - DNA polymerase
C. Helicase - DNA polymerase - Topoisomerase - Single stranded binding proteins
D. Helicase - Topoisomerase - DNA polymerase - Single stranded binding proteins
Answer» B. Helicase - single stranded binding proteins -Topoisomerase - DNA polymerase
29.

Which of the following enzymes can detect and correct the wrong inserted base during DNA replication ?

A. DNA polymerase - I
B. DNA polymerase - II
C. Primase
D. Ligase
Answer» A. DNA polymerase - I
30.

Which one is a ribozyme ?

A. Helicase
B. Ribonuclease - P
C. Peptidyl transferase
D. Both (B) & (C)
Answer» D. Both (B) & (C)
31.

Which of the following pairs is not correctly matched ?

A. Recombinant DNA - DNA forming by union of segments of DNA from diffrent Sources
B. Purines - Nitrogenous bases Cytosine , thymine and Uracil
C. ATP - The principal energy carrying compound inthe cell
D. r-RNA - RNA molecules found in ribosomes
Answer» B. Purines - Nitrogenous bases Cytosine , thymine and Uracil
32.

Which one of the following pairs is correctly matched ?

A. Ribosomal RNA - Carries amino acids to the site of protein synthesis
B. Transcription - Process by which protein in synthesized
C. Translation - Process by which m RNA carries the information from nucleus to ribosomes
D. Anticodon - Site of t-RNA that binds to the m-RNA
Answer» D. Anticodon - Site of t-RNA that binds to the m-RNA
33.

Which is not the step of translation ?

A. Initiation
B. Replication
C. Elongation
D. Termination
Answer» B. Replication
34.

The enzyme amino acyl t-RNA-synthetase facilitates.

A. Joining two neighbouring amino acids on ribosomes
B. A doption of amino acids by a t-RNA molecule
C. Insertion of amino acyl t-RNA into the ribosome sites
D. Transfer of amino acyl t-RNA from the ribosomal ‘A’ site to ‘P’ site
Answer» B. A doption of amino acids by a t-RNA molecule
35.

state the anticodon of initiation codon of protein synthesis

A. UAC
B. UUU
C. CAU
D. AUG
Answer» A. UAC
36.

Which is the energy source for the process of elongation ?

A. ATP
B. GTP
C. Creatine-PO4
D. All the above
Answer» B. GTP
37.

What does a gene consist of ?

A. Promoter
B. Initiation site & termination site
C. coding sequence
D. All the above
Answer» D. All the above
38.

Name the enzymes needed for lactose Catabolism in E.coli ?

A. β - galactosidase, permease transacetylase
B. β - galactosidasee Lactase, transacetylase
C. β - galactosidase, lactase, permease
D. Lactase, permease, transacetylase
Answer» A. β - galactosidase, permease transacetylase
39.

What does operon contain ?

A. Regulator gene + promotor gene
B. Operator gene + structural gene
C. Regulator gene + promotor gene structural gene
D. Regulator gene + promotor gene + operator gene + structural gene
Answer» D. Regulator gene + promotor gene + operator gene + structural gene
40.

Select correct match with respect to lac-operon model ?

A. Active represser + inducer  Inactive repressor
B. Active repressor + corepressor  Inactive repressor
C. Inactive repressor + inducer  Active repressor
D. Inactive repressor + corepressor Active repressor
Answer» A. Active represser + inducer  Inactive repressor
41.

In relation of lac operon in E-coli , Which protein is not regulated by the repressor ?

A. Tryptophan
B. galactosidase
C. Lactose permease
D. Transacetylase
Answer» A. Tryptophan
42.

Which is not correct regarding the activity of helicase during DNA replication ?

A. Cuts hydrogen bomds
B. Requires ATP
C. separates DNA strands
D. Stabilizes single strands
Answer» C. separates DNA strands
43.

Which of the following enzyme is not produced by E. coli during lactose catabolism ?

A. β - galactosidase
B. Thioglactoside translacetylase
C. Lactose dehydrogenase
D. Lactose permease
Answer» C. Lactose dehydrogenase
44.

Which is the incorrect statement regarding HGP ?

A. HGP is an Indian scientific research project
B. In 1990, the Project was initiated
C. A working draft of the genome was announced in 2000
D. In February 2001, the analysis of the working draft was published
Answer» A. HGP is an Indian scientific research project
45.

Humans have approximately _____ times more genes than E.coli

A. 8
B. 15
C. 100
D. 50
Answer» D. 50
46.

Which of the following is not according to the chargoff’s rule ?

A. A = T
B. C = G
C. A + G = T + C
D. A + T / G + C =1
Answer» D. A + T / G + C =1
47.

Select the correct answer / answers from the following
1. Ligase :- Joins short segments of DNA together
2. DNA Polymerase :- cuts DNA at specific sequence
3. Helicase :- Breaks the hydrogen bonds between complementary pairs during DNA replication
4. Gyrase : - Joins weak hydrogen bonds between complementary pairs

A. 1, 2, 3 and 4, are corrent
B. 1 and 2 are correct, 3 and 4 are false
C. 1 and 3 are correct, 2 and 4 false
D. 1, 2, 3 are correct, 4 is false
Answer» C. 1 and 3 are correct, 2 and 4 false
48.

DNA replication in eukaryotes is

A. Unidirectional with many origin
B. Bidirectional with many origin
C. Unidirectional with single origin
D. Bidirectional with single origin
Answer» B. Bidirectional with many origin
49.

Aminoacids lysine, serine and arginine are coded by how many codons ?

A. 6, 4, and 3 respectively
B. 4, 2 and 2 respectively
C. 6 codons
D. 4 codons
Answer» C. 6 codons
50.

Which of the following amino acid is coded by 3 codons ?

A. serine
B. Proline
C. Tryptophan
D. Isoleucine
Answer» D. Isoleucine
51.

How many nucleotides make one okazaki segment in eukaryotes ?

A. 1000-1500
B. 100-200
C. 5000
D. Not fixed
Answer» A. 1000-1500
52.

Which of the following enzymes help in the process of formation of phosphodiester bond during reverse transcription ?

A. DNA - dependant RNA polymerase
B. DNA dependant DNA polymerase
C. RNA - dependant RNA polymerase
D. RNA - dependant DNA polymerase
Answer» D. RNA - dependant DNA polymerase
53.

The two strands of a DNA molecule are separted and one of them is analysed for its A + T / G + C ratio, This is found to be 0.2 What is the A + T / G + C ratio of the other strand

A. 0.02
B. 0.08
C. 0.8
D. 0.2
Answer» D. 0.2
54.

DNA replication in lagging strand of most of the eukaryotic organis ms is

A. conservative and continuous
B. semi conservative but discontinuous
C. conservative and semi - discontinuous
D. semi conservative but continuous
Answer» B. semi conservative but discontinuous
55.

How many bases consist in an average gene ?

A. 3, 00, 000
B. 3000
C. 4, 00, 000
D. 4000
Answer» B. 3000
56.

Match the following using salient features of Human genome project A B
(P) Less than 2 % genome (i) 3 billion nucleotide bases
(Q) Chromosome 1 (ii) 231 genes
(R) Y chromosome (iii) 2968 genes
(S) Human genome (iv) codes for protein

A. (P - iv) (Q - iii) ( R - ii) ( S - i)
B. ( P - iv) (Q - ii) (R - iii) (S - i)
C. (P - iv) (Q - i) (R - ii) (S - iii)
D. (P - i) (Q - iii) (R - iv) (S - ii)
Answer» A. (P - iv) (Q - iii) ( R - ii) ( S - i)
57.

State the use of moleculer medicine ?

A. Improves diagnosis of diseases
B. Used as gene theraphy
C. Used to understand several diseass like Alzheimer’s Parkinsons diseases etc.,
D. All the above
Answer» D. All the above
58.

Among the following which is used for separation of DNA fragments ?

A. centifugation
B. Cell fractionation
C. Cell homogenation
D. Electrophoresis
Answer» D. Electrophoresis
59.

In Which of the following DNA not directly involved ?

A. Repication
B. Transcription
C. Translation
D. Transformation
Answer» C. Translation
60.

Transcription begins when one of the following enzymes binds to promotor site.

A. DNA polymerase
B. RNA polymerase
C. helicase
D. Gyrase
Answer» B. RNA polymerase
61.

What dose A & B represent ?

A. Grycase , Helicase
B. Double Stranded Protein, Helicase
C. Helicase, Single strand binding protein
D. Topoisomerase Helicase
Answer» C. Helicase, Single strand binding protein
62.

State the process and mention the labelled protion.

A. Process of Translation - X-RNA Polymerase- Y-DNA Template- Z-m RNA Transcript
B. process of Transcription - X-RNA Polymerase- Y-DNA Template- Z- RNA Transcript
C. process of Translocation - X-DNA polymerase- Y- Template- Z- Transcript
D. Process of Transformation - X - DNA polymerase- Y - RNA template- Z - RNA transcript
Answer» B. process of Transcription - X-RNA Polymerase- Y-DNA Template- Z- RNA Transcript
63.

What do P, Q, R and S regions of t RNA ?

A. P - Anticodon loop
B. P. D LoopQ - Variable loop Q - T ψ c loopR - T ψ c loop R - Variable loopS - D Loop S - Anticodon loop
C. P - T ψ c loop
D. P - Anticodon LoopQ - D loop Q - T ψ c loop R - Anticodon loop R - D loop S - Variable loop S - Variable loop
Answer» A. P - Anticodon loop
64.

Which state is represent by the above model

A. Repressed state of lac operon
B. Inactive state of Lac operon
C. Active state of Lac operon
D. Induced state of Lac operon
Answer» A. Repressed state of lac operon
65.

What does X replesent in the above diagram

A. Released polypeptide chain
B. Released 3D protein molecule
C. Released secondary protein
D. Released tertiary protein
Answer» A. Released polypeptide chain
66.

What does ‘X’ represent

A. gene
B. segment of DNA
C. seqment of DNA coding for specific protein
D. Both A & C
Answer» D. Both A & C
67.

Write the codon for the anticodon on the t - RNA

A. AGU
B. UGU
C. UGA
D. ACU
Answer» A. AGU
68.

In the genetic code dictionary how many codons are used to code for all the 20 essential amino-acids? (AIPMT - 2003)

A. 20
B. 64
C. 61
D. 60
Answer» B. 64
69.

What would happen if in a gene encoding polypeptide of 50 aminoacids 25th codon (UAU) is mutated to UAA ? (AIPMT - 2003)

A. A Polypeptide of 24 aminoacids will be formed
B. Two polypeptides of 24 and 25 aminoacids will be formed
C. A polypeptide of 49 aminoacids will be formed
D. A polypeptide of 25 aminoacids will be formed
Answer» A. A Polypeptide of 24 aminoacids will be formed
70.

Which one of the following triplet codes, is correctly matched with its specificity for an aminoacid in protein synthesis or as ‘start’ or ‘stop’ codon ?

A. UCG - start
B. UUU - stop
C. UGU - Leucine
D. UAC - Tyrosine
Answer» D. UAC - Tyrosine
71.

What does “Lac” refer to in what we call the lac operon ? (AIPMT - 2003)

A. Lactose
B. Lactase
C. Lac insect
D. The number 1,00,000
Answer» A. Lactose
72.

The following ratio is generally constant for a given species (AIPMT - 2004)

A. A + G / C + T
B. T + C / G + A
C. G + C / A + T
D. A + C / T + G
Answer» C. G + C / A + T
73.

During transcription if the nucleotide sequence of the DNA strand that is being coded is ATACG then the nucleotide sequence in the m RNA would be (AIPMT - 2004)

A. TATGC
B. T C T G G
C. U A U G C
D. U A T G C
Answer» C. U A U G C
74.

Which one of the following makes use of RNA template to synthesize DNA (AIPMT - 2005)

A. DNA polymerase
B. RNA polymerase
C. Reverse transcriptase
D. DNA dependant RNA polymerase
Answer» C. Reverse transcriptase
75.

Protein synthesis is an animal cell occurs (AIPMT - 2005)

A. only on the ribosomes present in cytosol
B. only on ribosomes attached to the nuclear envelope and endoplasmic reticulum
C. On ribosomes present in the nucleolus as well as in cytoplasm
D. on ribosomes present in cytoplasm as well as in mitochondria
Answer» D. on ribosomes present in cytoplasm as well as in mitochondria
76.

E coli cells with a mutant z gene of the lac operon cannot grow in medium containing only lactose as the source of energy because (AIPMT - 2005)

A. the lac operon is constitutively active in these cells
B. they cannot synthesize functional beta galactosidase
C. in the presence of glucose E Coli cell do not utilize lactose
D. they cannot transport lactose from the medium into the cell
Answer» B. they cannot synthesize functional beta galactosidase
77.

Aminoacid Sequence in protein synthesis is decided by the sequence of (AIPMT - 2006)

A. r RNA
B. t- RNA
C. m RNA
D. c DNA
Answer» C. m RNA
78.

Antiparallel strands of a DNA molecule means that (AIPMT - 2006)

A. One strand turns clockwise
B. One strand turns anticlockwise
C. The phosphate groups of two DNA strands , at their ends share the same position
D. The phosphate groups at the start of two DNA strands are is opposite position (Pole).
Answer» D. The phosphate groups at the start of two DNA strands are is opposite position (Pole).
79.

Polysome is formed by (AIPMT - 2008)

A. a ribosome with several subunits
B. ribosomes attached to each other in a linear arrangement
C. several ribosomes attached to a single m RNA
D. many ribosomes attached to a strand of endoplasmic reticulum
Answer» C. several ribosomes attached to a single m RNA
80.

Whose experiments cracked the DNA and discovered unequivocally that a genetic code is a “triplet” (AIPMT - 2009)

A. Hershey and chase
B. Morgan and sturtevant
C. Beadle and Tatum
D. Nirenberg and Mathai
Answer» D. Nirenberg and Mathai
81.

Select the two correct statement out of the four statement given below about “ Lac opern”(AIPMT-2010)
(i) Glucose or galactose may bind with the repressor and inactivate it
(ii) In the absence of lactose the repressor bind with the operator region
(iii) The Z -gene codes for permease
(iv) This was elucidated by Francois Jacob and Jacques Monod
The correct statements are

A. ii and iii
B. i and iii
C. ii and iv
D. i and ii
Answer» C. ii and iv
82.

Which one of the following does not follow the central dogma of molecular biology ? (AIPMT - 2010)

A. Pea
B. Mucor
C. Chlamydomonas
D. HIV
Answer» D. HIV
83.

The lac opern consists of (AIPMT - 2010)

A. four regulatory genes only
B. One regulatory gene and three structural genes
C. Two regulatory genes and three structural genes
D. three regulatory genes and three structural genes
Answer» C. Two regulatory genes and three structural genes
84.

The 31 - 51Phosphodiester linkages inside a potynucleotide chain serve to join AIPMT - 2010)

A. One DNA strand with the other DNA strand
B. One nucleoside with another nucleoside
C. One nucleotide with another nucleotide
D. One nitrogen base with pentose sugar
Answer» C. One nucleotide with another nucleotide
85.

Ribosomal RNA is actively synthesized by

A. Lysosomes
B. nucleolus
C. nucleoplasm
D. ribosomes
Answer» B. nucleolus
86.

If one strand of DNA has the nitrogenous base sequence as ATCTG, What would be the complementary RNA strand Sequence (AIPMT - 2012)

A. TTAGU
B. UAGAC
C. AACTG
D. ATCGU
Answer» B. UAGAC
87.

Semiconservative DNA replication using 15 N was demonstrated by (AIIMS -1994)

A. Meselson
B. Taylor
C. Meselson and stahl
D. Hershey and chase
Answer» C. Meselson and stahl
88.

In operan concept, the operator gene combines with (AIIMS -1986)

A. Regulator protein to switch off structural gene transcription
B. Regulator protein to switch on structural gene transcription
C. Inducer to switch off structural gene transcription
D. Regulator gene to switch off structural gene transcription
Answer» A. Regulator protein to switch off structural gene transcription
89.

Termination of polypeptide chain is brought about by

A. UUG , UAG and UCG
B. UAA , UAG and UGA
C. UUG , UGC and UCA
D. UCG , GCG and ACC
Answer» B. UAA , UAG and UGA
90.

RNA that picks up specific aminoacid from amino acid pool of cytoplasm to carry it to ribosome during protein synthesis is

A. t RNA
B. m RNA
C. r RNA
D. g RNA
Answer» A. t RNA
91.

Correct sequenceof code transfer during polypeptide formation is (AIIMS -1999)

A. DNA , mRNA , t RNA and amino acids
B. DNA , t RNA , r RNA and m RNA
C. m RNA , t RNA , DNA and amino acids
D. r RNA , DNA ,m RNA and t RNA
Answer» A. DNA , mRNA , t RNA and amino acids
92.

Best method to determine paternity is

A. Protein analysis
B. chromosome counting
C. gene counting
D. DNA finger printing
Answer» D. DNA finger printing
93.

DNA is copied from m-RNA molecule with help of

A. Restriction enzyme
B. Reverse transcriptase
C. DNA polymerase
D. Adenosine deaminase
Answer» B. Reverse transcriptase
94.

Nucleotide arrangement in DNA can be seen by

A. X - ray crystallography
B. Electron microscope
C. Ultra centrifuge
D. Light microscope
Answer» A. X - ray crystallography
95.

Pneumococcus experiment proved that (AFMC - 1993)

A. Bacteria do not reproduce asexuallly
B. Bacteria undergo binary fission
C. DNA is genetic material
D. RNA may sometimes control a production of DNA and protein
Answer» C. DNA is genetic material
96.

A DNA nucletotide chain has A G C T T C G A sequence The nucleotide sequence of other chain would be

A. T C G A A G C T
B. G C T A A G C T (AFMC - 1993)
C. T A G C A T A T
D. G A T C C T A G
Answer» A. T C G A A G C T
97.

A functional unit in synthesis of protein is (MPPMT - 1994)

A. Lysosome
B. Peroxisome
C. Polysome
D. Dictyosome
Answer» C. Polysome
98.

VNTR is employed for (AMU - 2002)

A. Protoplasmic culture
B. DNA finger printing
C. Regulation of plant growth hormones
D. Enhancing photosynthesis in desert plant
Answer» B. DNA finger printing
99.

Out of 64 codons 61 code for 20 types of aminoacids It is due to (CBSE - 2002)

A. Overlapping genes
B. Degeneracy of genetic code
C. Wobbling of codons
D. Universality of codons
Answer» B. Degeneracy of genetic code
100.

Okazaki fragments are joined by enzyme (Kerala 2005)

A. DNA helix
B. DNA ligase
C. DNA polymerase II
D. RNA polymerase II
Answer» B. DNA ligase
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